X-Message-Number: 10699 Date: Wed, 04 Nov 1998 23:16:57 -0800 From: Brian Manning Delaney <> Subject: Re: Calorie Restriction References: <> To Thomas Donaldson, To continue this discussion when I said I was going to stop is definitely not going to help my reputation for arguing people into the ground , but.... well, maybe having started with this line will help a little. :) In your case, it should of course be "TRYING to argue someone into the ground". You're serious. I want you on my team! In all earnestness, I very much appreciate your rigor and interest in this topic, and, as I always say, I will buy someone his (his/her) weight in chocolate if he can convince I don't need to be on CR. (No, wait, I'll buy MYSELF my weight in chocolate -- hmm, make that HIS weight :) One of the things that's been helpful is that you're reminding me that "ancient" (as I call them) life span studies shouldn't be dismissed by dint of their age. They are often flawed, but can yield very useful morsels. A few quick (ha) points: > None of the strains I've seen used for aging > experiments suffers from any particular > characteristic disease, either --- though some > of the shortest lived strains, for instance, > have a strong tendency to die early of leukemia. The point I was making before about problems using short-lived strains in life-extension experiments can still apply even if the autopsies reveal multiple causes of death, and even if the strain is medium-lived, and not short-lived. There, though, my two human equivalent analogies wouldn't apply, to be sure. But there are plenty of genetic flaws which manifest themselves in a wide array of diseases, and which might be corrected by one agent. Congenital immune system weakness, for example, results in early death my many causes. This is why Walford has always insisted -- I think rightly -- that we can't make anti-aging claims about a life-extension regimen unless the study on which the claims are based is done on long-lived strains. A further condition I would add (the old Steve Harris quote addresses this) is that the experimental group actually live beyond the normal strain maximum life span. That is, bad husbandry on a long-lived strain might shorten lives in a way which some agent might _partially_ correct. You might find a max. life span gain in the expt. group in comparison with controls, but that doesn't mean it's slowing aging. Something from the day before: > If you actually read up on melatonin It's only the two particular studies you cited that I haven't read recently. Melatonin research I've followed keenly (though I'm not totally up to date). > you will > discover that it is among the drugs which may > mimic CR, at least in some respects. The reason > for this is that CR retards changes in the > pineal gland (which produces melatonin), and > thus with CR your melatonin remains relatively > high. I just got into this question in Sci.life-extension. Self-quoting is annoying but... SLE> For (just about) each instance of a postulation SLE> that the CR effect results from a change in SLE> parameter X, say a slowing of the age-associated SLE> decline in melatonin production [1], there's the SLE> rejoinder that such prevention is "ultimately" SLE> caused by a decrease in free-radical damage. (Of SLE> course it could be the result of better repair SLE> or neutralization, something quite different SLE> from fewer radicals being generated.) That is, SLE> the argument would go, pinealocytes are staying SLE> youthful longer because of reduced free radical SLE> damage. Of course one can easily test which is SLE> primary, it would at first seem, by looking to SLE> see whether cells elsewhere are also staying SLE> younger longer. And they are. But then the SLE> melatonin theorist says, "Ah but melatonin SLE> itself prevents free radical damage _throughout_ SLE> the body, so...." SLE> SLE> Thus the difficulty in nailing down the precise SLE> mechanism of CR's action. You've probably read this, but this was the reference: [1] Stokkan KA, et al. Food restriction retards aging of the pineal gland. Brain Res. 1991 Apr 5;545(1-2):66-72. PMID: 1713529; UI: 91316606. http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=1713529&form=6&db=m&Dopt=b The point -- I'm not saying you're taking issue with this -- is that we simply don't yet know the relation among the relevant variables yet. And: figuring it out is tricky. Some melatonin researchers have, in my view prematurely, argued that CR works by dint of its effect on the pineal gland. We simply can't yet conclude that. (Nevertheless, you've got me realizing that there are indeed a lot of no-brainer experiments that haven't been tried, that should be, even if I still think you underrate, or underrated, the CR research agenda. Recently discovered set-point regulators, for example, should be investigated with respect to CR. I do know one group working on this, but these things take time to complete, and publish....) > Pierpaoli is far from the only person who > has done lifespan experiments with melatonin. > There is a popular book which you might get > which discusses these various issues, RJ Reiter > and J Robinson, MELATONIN. Yeah, I've glanced at it. I believe in peer-review.... [....] > Pierpaoli has also written his own book on > melatonin, THE MELATONIN MIRACLE. This, too, > deserves a read, ESPECIALLY if you want to > criticise his experiments. Every time I reach for this book the title turns me off. But I trust your judgement. > One interesting experiment (which might --- or > might not --- show that melatonin and CR are > related) is to try CR on mice which lack a > pineal gland. Since some strains of mice also > make melatonin elsewhere, you'd want to make > sure that you don't do the experiment on such a > strain. I mentioned gland-mutilation AND'ed with CR previously. I owe you a reference 9and a couple others), and you'll get one after I go to the library. (It wasn't the pineal, though.) My recent move caused file-loss (temporary, I hope). > And I doubt very strongly that CR is the only > strategy available to us if we wish personally > to delay our aging. To be clear: I've been claiming _specifically_ that CR is the only strategy which we have good reason to believe slows aging appreciably. This leaves open 1) the possibility that agents we know about now _might_ work well but just need to be tested under better circumstances; and 2) the actuality that the evidence we DO have allows us to conclude with a significant probability that some other agents slow aging somewhat -- but not as much as CR. The evidence, even the guesswork, just isn't there. It's all the same team, Brian Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=10699