X-Message-Number: 10831
Date: Tue, 24 Nov 1998 11:37:46 -0800 (PST)
From: Doug Skrecky <>
Subject: rapid freezing of brain tissue

Authors
  Nochlin D.  Mackenzie AP.  Bryant EM.  Norwood TH.  Sumi SM.
Institution
  Department of Pathology, University of Washington School of Medicine, Seattle
  89195-0001.
Title
  A simple method of rapid freezing adequately preserves brain
  tissue for immunocytochemistry, light and electron microscopic examination.
Source
  Acta Neuropathologica.  86(6):645-50, 1993.
Abstract
  A simple and reproducible method for cryopreservation of
  brain tissue from patients with Alzheimer's disease is
  described. Fresh brain slices (1 cm thick) obtained less
  than 6 h postmortem are placed in sealed plastic bags, sandwiched between
  0.3-cm-thick aluminium sheets, and frozen by placing the entire "sandwich"
  between layers of dry ice pellets. The frozen brain slices
  are stored at -85 degrees C. Specific anatomic areas can be retrieved at any
  time for light and electron microscopic, immunocytochemical, autoradiographic
  and neurochemical studies.

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