X-Message-Number: 10831 Date: Tue, 24 Nov 1998 11:37:46 -0800 (PST) From: Doug Skrecky <> Subject: rapid freezing of brain tissue Authors Nochlin D. Mackenzie AP. Bryant EM. Norwood TH. Sumi SM. Institution Department of Pathology, University of Washington School of Medicine, Seattle 89195-0001. Title A simple method of rapid freezing adequately preserves brain tissue for immunocytochemistry, light and electron microscopic examination. Source Acta Neuropathologica. 86(6):645-50, 1993. Abstract A simple and reproducible method for cryopreservation of brain tissue from patients with Alzheimer's disease is described. Fresh brain slices (1 cm thick) obtained less than 6 h postmortem are placed in sealed plastic bags, sandwiched between 0.3-cm-thick aluminium sheets, and frozen by placing the entire "sandwich" between layers of dry ice pellets. The frozen brain slices are stored at -85 degrees C. Specific anatomic areas can be retrieved at any time for light and electron microscopic, immunocytochemical, autoradiographic and neurochemical studies. Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=10831