X-Message-Number: 10835
Date: Wed, 25 Nov 1998 10:28:28 -0800 (PST)
From: Doug Skrecky <>
Subject: freeze storage of brains

Authors
  Dong JF.  Detta A.  Hitchcock ER.
Institution
  Department of Neurosurgery, University of Birmingham, Smethwicks, UK.
Title
  Susceptibility of human foetal brain tissue to cool- and
  freeze-storage.
Source
  Brain Research.  621(2):242-8, 1993 Sep 10.
Abstract
  Human second trimester foetal brain tissue was stored for a
  period of 1-6 weeks under various conditions in an attempt to evaluate
  factors influencing its susceptibility (cell loss) and survivability.
  Post-storage viability of mesencephalon, striatum, cerebellum and occipital
  cortex was assessed by a protocol combining vital staining with cell density
  counts so that tissue viability and cell loss could be evaluated
  simultaneously; tissue survivability was evaluated by cell culture. A
  significant amount of cell loss occurred after 24 h storage at room
  temperature, after one week at 4 degrees C and by two weeks at -20 degrees C
  in all structures; storage at -196 degrees C resulted in 17-21% cell loss at
  the end of a 6 week period. At -20 degrees C the cryoprotective effect of 20%
  FCS was equivalent to that of 15% FCS + 7% DMSO combined, suggesting
  potential use of serum in replacement of chemical additives. The procedure
  for removal of DMSO was critical to cell viability and survivability: single
  step dilution led to 27-39% greater cell loss than slow, multi-step
  dilutions. In comparison to fresh, non-stored tissue, immunocytochemical
  characterization of in vitro propagated stored tissue revealed no changes in
  the populations of major constituent cell types including neurones,
  dopaminergic neurones, glial and fibroblast cells. These results provide
  information on possible conditions under which transplant tissue can be
  satisfactorily stored depending on the prevailing requirements.

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