X-Message-Number: 11611
Date: Sat, 24 Apr 1999 21:32:16 -0700 (PDT)
From: Doug Skrecky <>
Subject: electrochemical fixation techniques

Citations: 1-2
  Garzon-De La Mora P.  Garcia-Estrada J.  Ballesteros-Guadarrama A. 
  Navarro-Ruiz A.  De Jesus Macias-Comparan J.  Murillo-Leano M. 
  Casillas-Ochoa J.  Pena-Moreno P.
  Division de Bioquimica-Farmacologica, Instituto Mexicano del Seguro Social,
  Guadalajara, Mexico.
  Electrochemical fixation
  techniques. I. Electrochemical
  fixation of human brain.
  Archives of Medical Research.  27(1):37-42, 1996 Spring.
  An electrochemical brain fixation procedure
  (EBFP) to treat brains excised from human cadavers is described thoroughly.
  It is as precise as any other similar method currently available. However, it
  takes only as much as 36 h to completion instead of the much longer lapses
  required by immersion in formaldehyde. Actions were taken to secure that it
  is not a source of artifacts of any kind, neither neurons nor glia or blood
  vessels. It is, therefore, amenable to be used as a valuable research and
  teaching tool. Other advantages are that it does not pose any health hazard,
  is money- and time-saving, and cuts down on equipment and facilities.

  Garcia-Estrada J.  Garzon-de la Mora P.  Ballesteros-Guadarrama A. 
  Macias-Comparan JD.  Murillo-Leano M.  Navarro-Ruiz A.  Casillas-Ochoa J. 
  Pena-Moreno P.
  Division de Bioquimica-Fannacologica, Centro de Investigacion de Occidente
  (CIBO), Instituto Mexicano del Seguro Social, Guadalajara, Jal., Mexico.
  Electrochemical fixation
  techniques. II. Electrochemical dog body
  fixation. Histological study.
  Archives of Medical Research.  27(2):127-32, 1996 Summer.
  This is the first attempt to harden all organs of a body together without
  excising them. This process was accomplished in bottom-belted,
  gastrointestinal (GI) or intravenously (i.v.) catheterized dog cadavers so as
  to influx an electrolytic solution containing formaldehyde (ESF). The i.v.
  influx of ESF was found to be the best perfusion pathway. After 48 h of
  immersion in ESF, 24 h current time of 17.5 A of current intensity, 24
  degrees to 56 degrees C, we ended up with thoroughly fixed dog cadavers that
  were wrapped with ethyl alcohol:glycerol gauzes and stored in plastic bags at
  room temperature. Optical microscopy of every sliced tissue showed normal
  blood vessels, neurons, glial and Purkinje cells and their nuclei of brain
  and cerebellum, respectively. Cardiac muscle fibers were of normal
  appearance. Kidney Bowman's capsule and space were found to be normal except
  for vacuolarly degenerated tubules. Small intestine showed normal epithelial
  cells and crypts of Lieberkuhn. In liver, sinusoids were normally arrayed but
  showed vacuolar cell degeneration. Herein a method to attain an
  electrochemical whole body fixation is

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