X-Message-Number: 11795
Date: Sat, 22 May 1999 00:36:02 -0700 (PDT)
From: Doug Skrecky <>
Subject: ethylene glycol and brain preservation

Authors
  Watson RE Jr.  Wiegand SJ.  Clough RW.  Hoffman GE.
Title
  Use of cryoprotectant to maintain long-term peptide immunoreactivity and
  tissue morphology [published erratum appears in Peptides 1986
  May-Jun;7(3):545].
Source
  Peptides.  7(1):155-9, 1986 Jan-Feb.
Abstract
  Use of an ethylene glycol based
  cryoprotectant solution has been found to be effective for the long-term
  storage of brain tissue either in block form or as freely
  floating sections prior to immunocytochemical processing. Storage of tissue
  in the solution at -20 degrees C or 4 degrees C for up to 3 months produced
  no adverse effects upon tissue morphology, nor was LHRH immunoreactivity
  diminished or accompanied by elevated non-specific staining. Furthermore,
  ultrastructural analysis of cryoprotected tissue revealed excellent
  preservation of cellular morphology. It is anticipated that this method can
  find use when it is necessary or desirable for the investigator to retain
  tissue for later immunocytochemical or electron microscopic processing.

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