X-Message-Number: 11925
Date: Tue, 8 Jun 1999 13:28:07 -0700 (PDT)
From: Doug Skrecky <>
Subject: effect of xylitol on freeze-drying injury

  Krallish I.  Jeppsson H.  Rapoport A.  Hahn-Hagerdal B.
  Institute of Microbiology and Biotechnology, Latvian University, Riga,
  Effect of xylitol and
  trehalose on dry resistance of yeasts.
  Applied Microbiology & Biotechnology.  47(4):447-51, 1997 Apr.
  The effects of dehydration/rehydration on two strains of
  Saccharomyces cerevisiae: S600, a metabolically engineered xylose-utilising
  strain, and H158, the non-xylose-utilising host strain; and on the naturally
  xylose-utilising yeast Pachysolen tannophilus CBS 4044, were compared after
  glucose and xylose utilisation respectively. The yeast strains differed in
  their ability to excrete and accumulate intracellular
  xylitol. A high intracellular xylitol
  content before and after dehydration coincided with a higher viability after
  a dehydration/ rehydration cycle. The intracellular
  trehalose content increased during dehydration in all three
  yeast strains, but this did not correspond to enhanced cell viability after
  dehydration/rehydration. The results are discussed in relation to the ability
  of xylitol and trehalose to structure

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