X-Message-Number: 11925 Date: Tue, 8 Jun 1999 13:28:07 -0700 (PDT) From: Doug Skrecky <> Subject: effect of xylitol on freeze-drying injury Authors Krallish I. Jeppsson H. Rapoport A. Hahn-Hagerdal B. Institution Institute of Microbiology and Biotechnology, Latvian University, Riga, Latvia. Title Effect of xylitol and trehalose on dry resistance of yeasts. Source Applied Microbiology & Biotechnology. 47(4):447-51, 1997 Apr. Abstract The effects of dehydration/rehydration on two strains of Saccharomyces cerevisiae: S600, a metabolically engineered xylose-utilising strain, and H158, the non-xylose-utilising host strain; and on the naturally xylose-utilising yeast Pachysolen tannophilus CBS 4044, were compared after glucose and xylose utilisation respectively. The yeast strains differed in their ability to excrete and accumulate intracellular xylitol. A high intracellular xylitol content before and after dehydration coincided with a higher viability after a dehydration/ rehydration cycle. The intracellular trehalose content increased during dehydration in all three yeast strains, but this did not correspond to enhanced cell viability after dehydration/rehydration. The results are discussed in relation to the ability of xylitol and trehalose to structure water. Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=11925