X-Message-Number: 11949
Date: Sat, 12 Jun 1999 09:15:20 -0700 (PDT)
From: Doug Skrecky <>
Subject: glycerol and blood-brain barrier

  Shah MV.  Audus KL.  Borchardt RT.
  Department of Pharmaceutical Chemistry, University of Kansas, Lawrence 66045.
  The application of bovine
  brain microvessel endothelial-cell monolayers grown onto
  polycarbonate membranes in vitro to estimate the potential
  permeability of solutes through the blood-brain barrier.
  Pharmaceutical Research.  6(7):624-7, 1989 Jul.
  Previously our laboratory (Rim et al., Int. J. Pharm. 32:79-84. 1986)
  described an in vitro blood-brain barrier (BBB) model consisting of cultured
  bovine brain microvessel endothelial cells
  (BMECs) grown onto regenerated cellulose acetate membranes. However,
  the utility of this in vitro BBB model system was limited
  because the regenerated cellulose acetate membrane and not
  the monolayer of bovine BMECs was rate
  limiting for the permeability of very lipophilic compounds.
  Therefore, in this study we have evaluated polycarbonate
  membranes as supports for growing bovine BMECs and for
  conducting in vitro drug permeability studies. Bovine BMECs
  were cultured on collagen-coated polycarbonate membranes (13-mm diameter,
  12-microns pore size) which were then mounted into
  side-by-side diffusion cells for transport studies. The
  permeabilities of a series of solutes of varying lipophilicity (progesterone,
  estrone, testosterone, haloperidol, propranolol, antipyrine, caffeine, urea,
  acyclovir, ganciclovir, ribavirin, and glycerol) were determined and an
  excellent correlation (r = 0.97) was established between the
  permeability coefficients of the solutes and
  their log partition coefficients (PC)/(MW)1/2.
  These results suggest that bovine BMECs
  cultured onto polycarbonate membranes can be used as an in vitro model system
  for estimating the potential permeability of a solute
  through the BBB in vivo.

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