X-Message-Number: 12170
Date: Wed, 21 Jul 1999 19:30:16 -0700
From: Yuri Pichugin <>
Subject: cryoprotectants

Here is an abstract of my review article on cryoprotectants. I can send
it as an attached Word file to who is interested in this theme. I need
an e-mail address of a French cryobiologist, Dr. Boutron. If somebody
have the address, please, send it to me.

PROBLEMS OF CRYOBIOLOGY 1993, No. 2, p. 3-9
(A scientific journal published by the Institute for Problems of
Cryobiology and Cryomedicine, Kharkov, the Ukraine)

Pichugin Yu.I

      RESULTS AND PERSPECTIVES IN SEARCHING
      OF NEW ENDOCELLULAR CRYOPROTECTANTS

Summarizing own results and literature data on the problems of
researching new endocellular cryoprotectants, the known permeable
cryoprotective agents have been singled out into the type of
endocellular hydrophilic cryoprotectants. It has
been concluded that the search of new effective cryoprotectants
within this type is not promising. Basing on the dependence
between the citotoxicity and cryoprotective efficiency of diols
and their structure, and physico-chemical properties, a possible
mechanism of action of agents during cryopreservation of
cells is considered. The number of effective endocellular
hydrophilic cryoprotectants is limited by twenty derivatives
of the following three chemical classes: polyols (diols,
glycerol), amides, and sulfoxides. Further progress in
cryopreservation is associated with the search of new
cryoprotectant types or physical effects on cells with the
purpose of increasing their resistance to freezing.

Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=12170