X-Message-Number: 12358
Date: Wed, 1 Sep 1999 07:06:55 -0700 (PDT)
From: Doug Skrecky <>
Subject: long-term storage at -80 C

  Galmes A.  Besalduch J.  Bargay J.  Novo A.  Morey M.  Guerra JM.  Duran MA.
  Servicio de Hematologia y Hemoterapia, Hospital Universitario Son Dureta,
  Palma De Mallorca, Spain.
  Long-term storage at -80 degrees C of
  hematopoietic progenitor cells with 5-percent dimethyl sulfoxide as the sole
  Transfusion.  39(1):70-3, 1999 Jan.
  BACKGROUND: Hematopoietic progenitor cells (HPCs) can be cryopreserved and
  stored below -120 degrees C in liquid nitrogen or at -80 degrees C in
  mechanical freezers. STUDY DESIGN AND METHODS: The feasibility of
  long-term storage of HPCs at -80 degrees C
  was investigated. The studies included a comparison of 5- and 10-percent
  dimethyl sulfoxide (DMSO) as cryoprotectant at various lengths of
  storage time. Mononuclear cell (MNC) recovery and viability
  and colony-forming unit-granulocyte-macrophage (CFU-GM) and burst-forming
  unit-erythroid (BFU-E) recovery assays were performed. The peripheral blood
  HPCs of 24 consecutive patients included in the program of autologous
  transplantation were studied. RESULTS: The MNC viability decreased
  progressively with the length of time from cryopreservation, reaching 32
  percent after 31 months of storage. The recovery rates of
  CFU-GM and BFU-E also decreased progressively with the duration of frozen
  storage, to 50 and 43.5 percent, respectively, after 12
  months and to 0 percent (both) after 24 months. At 6 months of
  storage, MNC viability was 80 percent, and CFU-GM and BFU-E
  recovery was 63.5 and 80.5 percent, respectively. There were no differences
  between MNCs cryopreserved with 5- or 10-percent DMSO in terms of cell
  viability. There were no differences between CFU-GM recovery or BFU-E
  recovery from HPCs cryopreserved in 5- or 10-percent DMSO. Patients given
  HPCs stored in these conditions for periods ranging between 123 and 202 days
  showed a complete and rapid hematologic recovery. CONCLUSION: HPCs can be
  cryopreserved at -80 degrees C with 5-percent DMSO and stored at -80 degrees
  C no longer than 6 months. A 5-percent DMSO concentration is comparable to a
  with 10-percent concentration in terms of recovery and MNC viability.

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