X-Message-Number: 12443
From: 
Date: Mon, 20 Sep 1999 14:08:22 EDT
Subject: 21CM patent

Go to www.uspto.gov to access the full text of the patent (Sep.14) assigned 
to 21st Century Medicine Inc. Oddly enough, the inventors listed do not 
include Greg Fahy; they are Brian G. Wowk, Michael G. Federowicz ["Darwin"], 
Sandra R. Russell, and Steven B. Harris. It runs about 18 printed pages.

Naturally, we will investigate the procedures and substances mentioned.

There are many interesting features in the patent, not least the structure of 
claims. The claims start broadly, including any alkoxylated organic compound 
in a concentration sufficient to permit vitrification and cooling until 
vitrified; and the application of this to any organ, tissue, or animal. More 
specific claims relate to use of 2-methoxyethanol and 
3-methoxy-1,2-propanediol.

The inventors acknowledge that glycol ethers have been used before to 
preserve embryos and cell suspensions, but believe they have priority in 
application to tissues, organs, and animals. Obviously, there are potentially 
interesting questions in patent law--both as to the relation between these 
inventors and prior users of glycol ethers, and between these inventors and 
later users of new variations and applications.

"Summary" of the invention mentions perfusing an inert fluid through the 
vascular system and controlling the temperature of the fluid; and a class of 
new glycol ether CPAs. Oddly enough, the Claims do not (to my legally 
untutored mind) appear to make any direct or specific reference to cooling 
methods, even though in the "Summary" that seems important.

"Glycol ethers" are understood to include compounds containing alkoxy--and 
particularly methoxy--functional groups. Included are alkoxylated alkanes and 
 alkoxylated alcohols and polyols, with several sub-groups given.

For some organs, cooling and rewarming rates can exceed 100 degC per minute. 
[This seems to suggest possible use with other CPAs, such as amides.]

"Large animals can be perfused wit high concentrations of  glycol ethers near 
0 C with rapid equilibration, no dehydration, no edema or other visible 
evidence of toxic effects. Histologic preservation is excellent at 
microscopic and ultrasturctural levels." 

References include Skrecky's proposal in Cryonet #5174, 1995; CRYONICS: 
REACHING FOR TOMORROW (Wowk & Darwin, 1991; and THE PROSPECT OF IMMORTALITY, 
1964; and a Darwin/Hixon piece in CRYONICS July 1984, as well as various 
patents and publications in recognized journals.

I have not found any patent entries under "ice blockers," although the 
instant patent says that suppression of ice formation is an important 
feature. I believe 21CM has a different class of substances it calls ice 
blockers, acting through a different mechanism.

The "Conclusion" mentions unprecedentedly high rates of cooling and 
rewarming,;and the advantages of the new CPAs, including penetration and low 
viscosity, rapid equilibration, ice inhibition, minimization of toxicity, and 
glass forming properties.  

Congratulations to all the inventors involved. Lots of work ahead.

Robert Ettinger
Cryonics Institute
Immortalist Society
http://www.cryonics.org 

Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=12443