X-Message-Number: 12461
Date: Sat, 25 Sep 1999 17:36:37 -0400
From: Jan Coetzee <>
Subject: No more LN?

RNAlater 

TISSUE STORAGE:RNA STABILIZATION SOLUTION


    Simplifies sample collection - one reagent immediately renders
RNases
    within tissue inactive
    More freedom - no need to freeze samples in liquid nitrogen or rush
    samples back to the lab freezer
    Eliminates the need to freeze and grind most tissue samples
    Perfect for tissue collection "in the field"
    Flexible tissue storage - RNA is stable for 1 day at 37 C, 1 week at
25 C,
    1 month at 4 C or indefinitely at -20 C
    Compatible with many RNA isolation procedures including all of
    Ambion's RNA isolation kits

WHAT IS RNAlater?
RNAlater  (patent pending) is an aqueous, non-toxic tissue storage
reagent that
stabilizes and protects cellular RNA in intact, unfrozen tissue samples.
RNAlater
eliminates the need to immediately process tissue samples or to freeze
samples
in liquid nitrogen for later processing. Tissue pieces can be harvested
and
submerged in RNAlater for storage without jeopardizing the quality or
quantity
of RNA obtained after subsequent RNA isolation.

HOW DO YOU USE RNAlater?
RNAlater has been tested on a variety of mammalian tissues, plants, E.
coli,
Xenopus, fish and Drosophila. The dissected tissue is simply submerged
in
approximately 5 volumes of RNAlater (e.g., a 0.5 g sample requires about
2.5 ml
of RNAlater) at room temperature. The solution permeates the cells,
stabilizing
the RNA. The sample can then be stored at -20 C indefinitely (the tissue
does not
freeze), at 4 C for up to a month or at 25 C for up to a week. The
tissue is later
removed from the solution for RNA isolation. Most tissues can be
transferred
directly to a lysis buffer and homogenized, however, some tissues (i.e.,
bone,
hard tumors) may require freezing in liquid nitrogen and grinding.
Samples
stored in RNAlater can be shipped on wet ice or even at room temperature
if
shipped overnight.

==================================================

Maybe they will develop a cell stabilizer that can stabilize the whole
cell in order to store tissue at -20 c.

J.C.

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