X-Message-Number: 12754
From: "Igor Artyuhov" <>
Subject: More on Ken Diller's experiments (Re: #12687)
Date: Tue, 9 Nov 1999 17:24:12 +0300

Kenneth R. Diller (Sc.D., Director, Biomedical Engineering Program, The
University of Texas at Austin) has kindly answered to my questions. Since
his answers may be of interest for other CryoNet members, I post them here:

>>>

>1. What was the preservation temperature? Was it dry ice, liquid nitrogen
or
>something else?

We store in liquid nitrogen

>2. What was the amount of tissue preserved? If it was in slices, then what
>were there dimensions?

For pancreas, we store the islet in a suspension.

>3. What cryoprotective agents have You used?

Dimethyl sulfoxide is the CPA that has worked best for pancreas islets.

>4. What was the percentage of cells to survive?

I don't have this number.  It would be with our clinical collaborators.

>5. What are Your further plans? May be a Kidney?

We have done some work in support of a group in England working to store
kidneys.  It  is likely another decade until success may be achieved.

<<<

The other surgeon to graft frozen tissues is Dr. Kutluk Oktay at New York
Methodist Hospital, who has successfully transplanted a segment of ovary to
a woman after 3 years in liquid nitrogen (ovary, not woman):
http://news.bbc.co.uk/hi/english/health/newsid_285000/285274.stm ,
http://news.bbc.co.uk/hi/english/health/newsid_455000/455204.stm .

______________________
Merry Eternity to all,
Igor Artyuhov
ICQ #34602339

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