X-Message-Number: 12831
Date: Wed, 24 Nov 1999 07:53:57 -0800 (PST)
From: Doug Skrecky <>
Subject: lactalbumin protects against membrane disruption

Authors
  Ollero M.  Blanco TM.  Lopez-Perez MJ.  Cebrian Perez JA.
Institution
  Departamento de Bioquimica y Biologia Molecular, Facultad de Veterinaria,
  Zaragoza, Spain.
Title
  Surface changes associated
  with ram sperm
  cryopreservation revealed by counter-current distribution in an aqueous
  two-phase system. Effect of different cryoprotectants.
Source
  Journal of Chromatography B: Biomedical Applications.  680(1-2):157-64, 1996
  May 17.
Abstract
  Ram sperm was frozen in the presence of the
  most commonly used cryoprotectants. After thawing, the overall cell
  surface changes provoked by freezing were
  assessed by centrifugal counter-current distribution (CCCD). In addition,
  cell membrane integrity (viability) of all the treated sperm
  was estimated by fluorescent staining. Fresh and refrigerated
  sperm were used as controls. Our results show no improvement
  of the cooling-induced cell surface damage by freezing in
  the presence of bovine seminal plasma, proline, glycine-betaine and
  phosphatidylcholine. Better results were obtained with
  vitamin E and cholesterol. However, the best protective effects were found by
  employing seroalbumin and lactalbumin. Furthermore, freezing in the presence
  of bovine lactalbumin resulted in a good maintenance of the cellular
  viability and of the CCCD heterogeneity in respect to fresh cells.

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