X-Message-Number: 13059
Date: Wed, 5 Jan 2000 01:27:24 -0800 (PST)
From: Doug Skrecky <>
Subject: freezing of mammalian livers

  Rubinsky B.  Arav A.  Hong JS.  Lee CY.
  Department of Mechanical Engineering, University of California at Berkeley
  Freezing of mammalian
  livers with glycerol and antifreeze proteins.
  Biochemical & Biophysical Research Communications.  200(2):732-41, 1994 Apr
  We have tested a protocol that uses a new cryoprotective solution for
  preserving mammalian livers in a frozen
  state. This protocol is based upon our studies on the mechanisms that freeze
  tolerant animals use to survive freezing in nature. The
  cryoprotective solution contains glycerol and antifreeze proteins, both of
  which are found in freeze tolerant animals. Whole rat livers
  were frozen to -3 degrees C, maintained at that temperature for 6 hours and
  then warmed to 37 degrees C. Post thawing bile production and microscopic
  analysis of tissue slices were used to verify liver function and tissue
  morphology. We conclude that antifreeze proteins used in the concentrations
  chosen here have a protective effect on the whole liver during

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