X-Message-Number: 13063 From: "John Clark" <> Subject: Protein Antifreeze Date: Thu, 6 Jan 2000 00:42:36 -0500 I have no knowledge of the practical application of Cryonics so I'd like to ask those that do if the following is a nutty idea. As I understand it some otherwise promising cryoprotectants are rendered useless because they're too viscous to infuse at body temperature, much less during cool down. But what if you engineered a bacteria to produce the cryoprotectant and then infused the bacteria; wouldn't that make the viscosity of the substance irrelevant and as a bonus be dirt cheap too? I got to thinking about this when I read at http://www.doe.gov/mussels.htm about a super glue that mussels use to attach themselves to rocks. This viscous super strong adhesive turns hard as a rock in about 60 seconds and is made up of 5 proteins. Scientists have now cloned all five genes that make the proteins so soon they'll be able to make the stuff by the ton, before it took 10,000 mussels to produce one gram of the adhesive. I recall that in the August 21 1997 issue of Nature there is an article by Virginia K Walker and Peter L Davies about a protein in the cold resistant mealworm insect that is the most powerful known organic antifreeze, 100 times as potent as the one found in arctic fish. It lowers the freezing point but it also does something much more important, when things get really cold and ice does form it does so as smooth hexagonal disks, not as dangerous sharp edges and pointy spikes which is what you get without any antifreeze or even if you use the one from arctic fish. Since only one protein is involved not 5 I would imagine it would be easier make cryoprotectant than adhesive but to my knowledge this has not been done, probably because most think a new glue is far more valuable than a new cryoprotectant. John K Clark Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=13063