X-Message-Number: 13754
From: "Pierre Le Bert" <>
Subject: please unregister me out of the maling list
Date: Sat, 20 May 2000 23:10:54 +0200
> -----Message d'origine-----
> De: CryoNet [mailto:]
> Date: samedi 20 mai 2000 11:00
> :
> Objet: CryoNet #13747 - #13751
>
>
> CryoNet - Sat 20 May 2000
>
> #13747: CryoNet #13726 - #13731 [Jeffrey Soreff]
> #13748: Viable cultured neurons in hibernation storage for a
> month. [Doug Skrecky]
> #13749: Russian web poll on cryonics [Mikhail Soloviev]
> #13750: Please, no numeric subject lines! [Kennita Watson]
> #13751: Head out on the Highway [Stephen W. Bridge]
>
> ----------------------------------------------------------------------
>
> Message #13747
> Date: Fri, 19 May 2000 09:30:30 -0400
> From: Jeffrey Soreff <>
> Subject: CryoNet #13726 - #13731
>
> Thomas Donaldson wrote:
>
> >If I properly understand just what you're saying, it still won't
> >work. Chemical compounds consist of atoms linked together; that
> >linkage is by electrons. If you can use X-rays to identify atoms
> >from carbon onward, you still need some way to work out which atom
> >is chemically attached to which other atoms. Physical nearness isn't
> >enough evidence.
>
> While I have other concerns about X-ray probes, ambiguity about
> bonding isn't a major concern. If one knew the positions of all
> of the atoms (really the center of electron density from X-ray
> structure, which is close to the positions of the nucleii, but
> not quite...), yes it is true that there is still ambiguity about
> chemical bonding, but it is only important in rare cases.
> If we could guarantee that the electrons were in the ground state
> for the specific positions of the nucleii, then there _wouldn't_
> be ambiguity. That is the reason that one can represent molecular
> mechanics reasonably well by potential energy surface calculations.
> Specifying the positions of nucleii and requiring the electrons to
> be in the ground state almost always (barring things like spin
> orientation in free radicals) gives a unique electronic state.
> Now electronically excited states _do_ have different electron
> distributions and different potential energy surfaces, and they
> are important during transition states of reactions, but I doubt
> that a significant number of molecules in a biological system
> are in electronically excited states at any given time. The
> cases of electronically excited states that I am aware of in
> biological systems are things like singlet oxygen, which is toxic.
> Does anyone know of any persistent electronically excited
> molecule in a biological system which is required for normal
> function?
> Best wishes,
> -Jeffrey Soreff
>
> ----------------------------------------------------------------------
>
> Message #13748
> Date: Fri, 19 May 2000 07:00:05 -0700 (PDT)
> From: Doug Skrecky <>
> Subject: Viable cultured neurons in hibernation storage for a month.
>
> NeuroReport 7: 1509-1512 1996
> Brewer G., and Price P.
>
> Abstract: Neurobasal is a bicarbonate-buffered medium
> optimized for the growth of embryonic rat hippocampal
> neurons at pH 7.3 in 5% CO2. Neurons die within hours
> in this or in other 26 mM bicarbonate buffers when transferred
> to ambient CO2 (0.2%). Death is associated with a rapid
> rise in medium pH to 8.1. A new CO2-independent
> modification of Neurobasal (Hiberate E), when
> supplemented with B27, can maintain neuron viability
> for at least 2 days in ambient CO2. This same medium
> can also be used to store viable brain tissue for up to a
> month with refrigeration. These advances should
> facilitate studies of neuron physiology outside the
> incubator as well as storing and transporting neuronal tissue.
>
> Additional note by poster:
>
> Rat embryo hippocampi have 100% cell viability after
> 1 week of storage in Hibernate/B27. After 4 weeks this is
> reduced to 50% cellular viability.
>
> ----------------------------------------------------------------------
>
> Message #13749
> Date: Fri, 19 May 2000 23:35:07 +0400
> From: Mikhail Soloviev <>
> Subject: Russian web poll on cryonics
>
> There was a cryonics poll on a Russian web search site
> "Aport" (www.aport.ru). It was ordered by "Kommersant-
> Dengi" ("Businessman-Money"), one of the best Russian
> business weekly magazine. A journalist of this magazine
> prepares an article on cryonics (I and some other cryonicists
> help him). I think, he was an author of this poll.
>
> The question was:
> "Would you like to be frozen after your death? (Why)"
>
> If you click on "Why" you see the following help window:
>
> "The scientists, conducting cryonics research, state, that
> the body, frozen in liquid nitrogen according to all rules,
> can be stored hundreds years without change. And they don't
> exclude that the possibility to return this body to life will
> appear in the far future. In the West the body-freezing
> service is very popular. For example, there is "Cryonics
> Society" in the USA that people join before the death with
> the hope to be resurrected in the future. There were rumors
> that such known people as Salvador Dali and Walt Disney
> bequeathed to freeze their bodies after the death."
>
> Today the journalist sent me the preliminary results of
> this poll.
>
> Number of answers: 831
>
> Yes, of course:
> 93 (11.2%)
> Yes, if I will have money:
> 36 (4.3%)
> Maybe:
> 91 (11.0%)
> Rather no:
> 131 (15.8%)
> Never:
> 100 (12.0%)
> I'm very surprized by your question:
> 380 (45.7%)
>
>
> -- Mikhail Soloviev
>
> ----------------------------------------------------------------------
>
> Message #13750
> Date: Fri, 19 May 2000 12:58:00 +0000
> From: Kennita Watson <>
> Subject: Please, no numeric subject lines!
> References: <>
>
> CryoNet wrote:
> >
> > CryoNet - Fri 19 May 2000
> >
> > #13742: CryoNet #13726 - #13731 [Thomas Donaldson]
> > #13743: CryoNet #13732 - #13736 [Thomas Donaldson]
> > #13744: Embryos of Artemia franciscana survive four years
> of continuous [Doug Skrecky]
> > #13745: vote to be frozen (poll on a Russian web search
> site) [Mikhail Soloviev]
> > #13746: Re: Vote to be Frozen (Mikhail Soloviev) [Olaf Henny]
> ..
>
> Cutting and pasting the individual message's subject -- and
> quoting enough to give some context -- is very helpful to a
> busy digest-reader.
>
> Thanks,
> Kennita
> --
> Kennita Watson | I vote Libertarian.
> | Find out why.
> http://i.am/kennita | http://www.lp.org/intro
>
> ----------------------------------------------------------------------
>
> Message #13751
> Date: Sat, 20 May 2000 01:39:57 -0400
> From: "Stephen W. Bridge" <>
> Subject: Head out on the Highway
>
> This is a very funny satire site of "Two young ladies traveling
> cross-country with the cryogenically frozen head of Walt
> Sidney. Not to be confused with Walt Disney. "
>
> But WE can confuse it with Walt Disney. Some of the pictures of Walt's
> frozen head visiting Mount Rushmore, the Hoover Dam, etc. are
> quite bizarre
> and clever.
>
http://www.icontech.com/~lorinac/
Steve Bridge
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