X-Message-Number: 13956
Date: Thu, 15 Jun 2000 20:33:36 -0700 (PDT)
From: Doug Skrecky <>
Subject: mercuric fixation of brain

  Rapid method for fetal brain fixation.
  Journal of Clinical Pathology.  41(9):1019-20, 1988 Sep.
  A quicker alternative to the standard removal and fixation
  of brain tissue was sought. Whole fetal
  brains were fixed in situ using a mercuric based fixative.
  The subarachnoid space was perfused overnight with Heidenhain's Susa
  fixative. The following day the brains were removed from the
  cranium in the standard manner. After storage for three days in Susa's
  fixative the brain was sliced and processed, with excellent
  preservation of gross and microscopic architecture. The cost is only
  marginally higher than that of commercial formalin.

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