X-Message-Number: 14074
From: "Shane Brubaker" <>
Subject: Question on cyronics procedures
Date: Tue, 11 Jul 2000 16:15:28 PDT

  Hello.  I'm interested in cryonics and I was wondering if I could ask some 
questions about the procedures used.  Specifically, is there any fixative 
(such as glutaraldehyde) used in the perfusion solution.
  It is my understanding that fixation is necessary to preserve synapses.  
When freezing a rat brain for instance using either paraformaldehyde or a 
cryoprotectent solution alone, such as sucrose, the synapses dissolve and 
are not present when observed via electron microscopy -- only the cell 
bodies and major trunks of the dendrites are preserved.  Although this might 
make a traditional resuscitation more difficult, it would be more certain to 
preserve brain structure.  I am more concerned with the preservation, for 
instance for use in a mind uploading procedure, than I am with having my 
original body resuscitated.
  Is this done by anyone, and if not could I make a specific request to have 
it performed on me if I were to arrange suspension through one of the 
existing organizations?  I'd greatly appreciate any information you can give 
me.
  Any references in regard to the fixation procedures and level of brain 
preservation would also be welcome.

  Thank You,

Shane Brubaker
Email: 
Web Page: http://www.geocities.com/Area51/Rampart/7937/index.html
Online Resume: http://www.angelfire.com/or/shanebrubaker/resume.html
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