X-Message-Number: 14288 Date: Thu, 10 Aug 2000 14:59:49 -0700 (PDT) From: Doug Skrecky <> Subject: Crippling Vitrification In Message #14282 wrote: >BioPreservation is gone, CryoCare is on ice , the Prometheus Project never raised one cent , CryoSpan seems to be on its way out, and INC's perpetual underfunding seems to be starving it to death. < If vitrification research is the goal, why not just do some experiments? Not all experiments have to be expensive, with my fly freezer experiment being one example. (My next fly longevity update will have a little more on this.) With respect to vitrification, the main roadblock to success appears to be toxicity. A cheap experiment testing means for reducing this might run something as follows. Expose water fleas, hydras, or some other very small aquatic organism to various potentially protective additives in their water for a few days. Reduce temperature to close to freezing, and start loading cryoprotectant into a test tube containing some of the organisms. See if cryoprotectant tolerance is increased with any additive. If so get rich (maybe). Are there any grad students out there willing to do this sort of experiment for a modest fee? Is there anyone willing to provide funding? Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=14288