X-Message-Number: 14307 From: Date: Sun, 13 Aug 2000 18:58:39 EDT Subject: credit to Pichugin In all the euphoria about recent research results involving the INC, 21CM etc., there has (as far as I have noticed) been very little public acknowledgement of the role of Yuri Pichugin, who did much of the experimental work (and some of the theoretical work) that led to the optimistic pronouncements on brain cryopreservation at Alcor's Asilomar conference. For various reasons, certain people involved don't want direct attribution or direct quotations, but from highly placed or/and well informed sources I have been given to understand the following, summarized and paraphrased: Dr. Pichugin was the only cryobiologist that could be found--world-wide!--qualified and willing to work on the hippocampal slice project. (This is old news, of course.) The brain slice program is virtually indispensable for the larger whole-brain cryopreservation research. Dr. Pichugin created or mastered the experimental techniques after others had repeatedly tried and failed over a period of years. His skills may be unique and irreplaceable in the time frame of the next year or two at least. He developed data showing that a temperature different from that previously used at Alcor for cryoprotectants, including glycerol, is preferable, and that led to improved results reported in the recent suspension of F.M. Esfandiary by Alcor. He was formerly a professional cryobiologist at the world's largest cryobiology research laboratory in Kharkov, Ukraine, with extensive training in biochemistry. In one of his papers he anticipated the usefulness of methoxylated compounds developed at 21CM. He was plunked down in California in a moldy wreck of an abandoned laboratory, but managed to overcome the difficulties with very little help. Previously, with collaborators in Europe, working on contract for CI, he had demonstrated coordinated electrical activity in rabbit brain pieces perfused with glycerol and rewarmed from liquid nitrogen temperature. His work has implications not only for cryonics, but for neural tissue banks and for research in clinical medicine pertaining to Alzheimer's, Parkinson's, and other diseases. Robert Ettinger Cryonics Institute Immortalist Society http://www.cryonics.org Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=14307