X-Message-Number: 14337 Date: Wed, 02 Aug 2000 13:53:12 +0200 From: Klaus Sames <> Subject: Follicular cryoprotectants Hallo to persons concerned, I have a remark to: #14326: permeation of ovarian tissue with cryoprotectants [Doug Skrecky]. Its not by accident that ovarian follicles resist low temperature. Follicular fluid contains proteoglycans of high polimerization degree and high waterbinding capacity.. Proteoglycans are known to be natural cryoprotectants. In cryosections of cartilage tissue which is extremly rich in proteoglycans (around cells) we found no damage as compared to other tissues using light microscopy,. However, we have no electron microscopic findings. It would be interesting to study how the native cryoprotection interacts with the cryoprotection performed by scientists. With best regards, Klaus Sames Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=14337