X-Message-Number: 14833
Date: Fri, 3 Nov 2000 05:19:03 -0800 (PST)
From: Doug Skrecky <>
Subject: different sugars have different effects on hemolysis

  L-Sorbose but not D-tagatose induces hemolysis of dog
  erythrocytes in vitro.
  Regulatory Toxicology & Pharmacology.  29(2 Pt 2):S43-5, 1999 Apr.
  Previous investigations have demonstrated that L-sorbose
  induces hemolysis of dog erythrocytes. This effect is probably the
  consequence of an ATP depletion of the red blood cells subsequent to
  inhibition of hexokinase, and thus the glycolytic pathway, by
  sorbose 1-phosphate. In the present study, the
  susceptibility of dog erythrocytes to D-tagatose, a stereoisomer of
  L-sorbose, was examined. Washed dog erythrocytes were
  suspended in Hanks' balanced salt solution (HBSS, containing 5.6 mM glucose)
  with or without the addition of 0.6, 6, and 60 mM L-sorbose
  or D-tagatose, or in HBSS with total glucose concentrations of 5.6, 6 and 60
  mM D-glucose. After incubation for 24 h at 34 degrees C, the suspensions were
  centrifuged, and the percentage of hemolysis was determined by measuring the
  hemoglobin in the sediment and the supernatant. The amount of hemoglobin
  released in the medium did not differ significantly between the control
  (HBSS) and the test incubations with glucose or D-tagatose supplementation.
  In contrast, the addition of 6 and 60 mM L-sorbose resulted
  in significant hemolysis. At the low dose (0.6 mM),
  L-sorbose did not have an adverse effect. It is concluded
  that D-tagatose, unlike L-sorbose, does not have a hemolytic
  effect on canine erythrocytes. Copyright 1999 Academic Press.

  Additional note by poster:

    The abstract fails to mention that the small amount of hemolysis
  induced by glucose itself was reduced by about 50% when tagatose 
  was substituted. Tagatose is an interesting sugar, which has not 
  yet been tested as a substitute for glucose in cryopreservation
  solutions. Tagatose is known to inhibit iron induced free radical
  damage, and reduce glycation.

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