X-Message-Number: 15081
Date: Tue, 5 Dec 2000 09:30:45 -0800 (PST)
From: Doug Skrecky <>
Subject: neuroprotective effects of pyridoxal phosphate

  Protective effects of
  pyridoxal phosphate against glucose
  deprivation-induced damage in cultured hippocampal neurons.
  Journal of Neurochemistry.  68(6):2500-6, 1997 Jun.
  When hippocampal cultures were deprived of glucose, massive release of
  lactate dehydrogenase (LDH), an indicator of neuronal death, occurred via
  NMDA receptor activation. Addition of pyridoxal
  phosphate (PLP; 1 and 10 microM) inhibited this LDH release
  in a concentration-dependent manner. Prior exposure to PLP evoked more potent
  inhibitory effects on LDH release compared with those
  treated at the onset of glucose deprivation. Furthermore, PLP inhibited the
  reduction of intracellular content of pyruvate induced by glucose
  deprivation, which was accompanied by the reversal of intracellular ATP
  depletion. A noteworthy elevation of extracellular glutamate in response to
  glucose deprivation was completely reversed by addition of PLP.
  Aminooxyacetic acid, a potent inhibitor of PLP-dependent enzymes, antagonized
  the effects of PLP on LDH release, pyruvate production, and
  ATP formation. These results suggest that PLP protects neurons from glucose
  deprivation-induced damage by enhancing the formation of energy-yielding
  products and relieving extracellular load of glutamate. The observed
  phenomena further indicate that PLP might be used prophylactically against
  neuronal death induced by metabolic disorders.

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