X-Message-Number: 15087
Date: Wed, 6 Dec 2000 08:42:11 -0800 (PST)
From: Doug Skrecky <>
Subject: fructose-1,6-diphosphate enhances heart preservation

  Fructose-1,6-diphosphate and a glucose-free solution enhances functional
  recovery in hypothermic heart preservation.
  Journal of Heart & Lung Transplantation.  19(3):277-85, 2000 Mar.
  BACKGROUND: Fructose-1,6-diphosphate (FDP) has been shown to protect tissue
  during hypoxia under various ischemic conditions, including isolated heart
  perfusion. We tested the hypothesis that adding FDP to St. Thomas solution
  can extend hypothermic heart preservation time. METHODS: Sixteen adult
  Sprague-Dawley rats were used. Under general anesthesia, the hearts were
  removed and preserved at 4 degrees C in St. Thomas solution (30 ml/kg) for 12
  hours. FDP (5 mM) was added to the St. Thomas solution in the study group (n
  = 8), whereas no FDP was used in the control group (n = 10). The hearts were
  reperfused after 12 hours of preservation using a working heart model.
  RESULTS: In the study group, cardiac output ranged from 13.00 +/- 2.34 to
  17.66 +/- 1.71 ml/min, maximum aortic flow was 3.40 +/- 1.99 to 9.26 +/- 1.72
  ml/min, left ventricular stroke volume ranged from 0.074 +/- 0.014 to 0.092
  +/- 0.009 ml, left ventricular stroke work ranged from 6.22 +/- 0.39 to 7.95
  +/- 0.44 ml/mmHg, and maximum left ventricular generated power was 14.38 +/-
  2.94 to 20.16 +/- 2.49 Joules/min. All of these parameters were higher than
  those in the control group (p < 0.001). Coronary vascular resistance and
  myocardial tissue wet/dry weight ratio were lower in the study group than in
  the control group (p < 0.05).CONCLUSIONS: Heart function was better preserved
  when FDP was added to St. Thomas solution during hypothermic rat heart
  preservation. The mechanism is not totally clear, but enhancement of
  high-energy phosphate production during ischemia is possible. Key words:
  heart, procurement, hypothermia, fructose-1,6-diphosphate.

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