X-Message-Number: 15214 From: "ramzy ramzy" <> Subject: Reply to Vitrification or Message 15210 from Ettinger Date: Sat, 30 Dec 2000 19:07:15 -0000 There are two points: 1- The hot cryonics is required for vitrification. I agree but not at -130 C in the neighborhood of glass transition temperature because we need to stop most of the biochemical reactions in the neighborhood of -150 C. From few days: I suggested Liquid Krypton at about 119.8 K instead of Liquid N at 77.36 K (Message 15188). Why? We want to choose a cryogenic Liquid at its boiling point because any other refrigeration system have many problems now. We have inactive six elements: He, Ne, Ar, Kr, Xe, Rn (Nitrogen is active element with comparing of the previous elements). The boiling point of these elements (and even Nitrogen)is the nearest from the range (-130 to -150 C or 123 to 143 K) in case of Kr at its boiling point (119.8 K). 2- I agree with you in other point. Problem of toxicity of vitrifiable cryoprotectant agent is present also, but I can go so far. If I accept toxicity of CPA to avoid crystallization, then I can accept a toxic cryogenic liquid has appropriate boiling point to avoid cracking. Then, we must seek an alternative compound instead of Nitrogen Liquid even it is toxic? The principle in the two kinds of toxicity is that: The mechanical damage is worse than the chemical damage. Happy new year from Egypt for all members. Ramzy _________________________________________________________________________ Get Your Private, Free E-mail from MSN Hotmail at http://www.hotmail.com. Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=15214