X-Message-Number: 15259 Date: Thu, 4 Jan 2001 22:44:35 -0500 (EST) From: Charles Platt <> Subject: Re: trade-offs in choosing protocols On Thu, 4 Jan 2001 wrote: > I think that for the first paragraph, a citation might be helpful, not > necessarily to a peer-reviewed article (because cryobiology in the > year 2000 may have as many biases floating around as psychology in the > year 1850) but to some website or other place where we can take a look > at the original work. This would certainly make it more convenient > for readers. (And any way numbers can be meaningfully assigned to > `better' would of course help, as well.) A very reasonable request! Unfortunately, much of the work was reported in back issues of Cryonics magazine (Alcor's publication), which are not available online (so far as I can tell--please correct me if I'm wrong) even though someone did copy-type or use OCR to transfer all the text of most back issues to disk, back in the late 1980s or early 1990s. (Plea to Alcor: It would be a great public service if the text of these back issues were added to your web page.) However, I do have some materials at my disposal, which I will be happy to send to people by USMail. 1. CryoCare Report #4. This contains a report of a comparative study, in which some dog brains were perfused using the best-available glycerol-based protocol at that time, while others were perfused using a "simplified" protocol. Although the text doesn't say so (because we were trying to be nonconfrontational and polite, believe it or not), the "simplified" protocol was based on reports from CI of their work using sheep brains. In other words, the purpose of the research by Darwin, Harris, and others was to find out whether CI protocol produced results comparable to the more sophisticated protocol. Very high-quality reproductions of electron micrographs are included, with principal features (good and bad) annotated. You don't have to be a brain surgeon to interpret these pictures. For instance an ice hole, where all brain tissue has been displaced, shredded, and/or mangled, is pretty damned obvious. This should have a sobering effect even on the most hardcore nanotech believers--if they would actually bother to look, instead of daydreaming about "What I'll be able to do after the Singularity." 2. CryoCare Update #6. This contains the best available report of the September, 1998 conference at which personnel from 21CM described their vitrification work, including preservation of two rabbit brains that were rewarmed from -80 Celsius (approx) with unprecedentedly good results. Again, high-quality reproductions of electron micrographs are included, providing some validation for claims of a genuine breakthrough. 3. For those who are mindful of Robert Ettinger's recent warning against unwarranted optimism, I also offer CryoCare Report #10, which contains an exhaustive account of Olga Visser's unsuccessful attempt to resuscitate rat hearts that had been frozen in liquid nitrogen. This attempt took place at the 1997 Alcor technology festival. Please note that Alcor renounced its association with Visser later that year. I will send any or all of these source materials, at $2 each including postage, to anyone who wants them. Send your order to: Charles Platt, 9 Patchin Place, New York, NY 10011. Since I travel a lot, it may take 7 to 21 days for me to send you your order. > Now, i don't want to be unduly argumentative here, but one implication of > the first paragraph seems to be that morticians are unqualified to > operate machinery interfacing with a human body. This is hard to > believe---morticians not only have to go through a degree program > (correct me if i'm wrong), but they also handle deanimated bodies all > the time. Please feel free to be as argumentative as you like! It's true that the morticians I have met have been more open-minded than many of the doctors I have met, where cryonics is concerned; and as you say, they receive training. However, their priorities are totally different from ours. When they use an embalming pump, for instance, they are not concerned with monitoring the pressure to avoid bursting brain capillaries. To them, the patient is dead--period. To us, this is not necessarily true. For this reason, no mortician should operate on a cryonics patient unsupervised, and field work should be done using a mobile kit such as the one built by Hugh Hixon at Alcor, or the elaborate setup created by Mike Darwin when he was active in cryonics. Morticians clearly are not perfusionists; and with the best will in the world, they can screw up in very basic ways. A mortician I dealt with in New York, who was extremely willing to oblige, could not imagine that we _really_ needed so much ice to pack around a patient who was being prepared for air shipment to California. There was no way I could convey to him the importance of this. From his point of view, he just wanted to please an unusual customer who had a thing about ice. From my point of view, it was a vital matter of calculating the mass of ice that would be necessary to maintain near-freezing temperatures for a 7-hour (minimum) journey in summer temperatures, using a casket that contained virtually no insulation (we did not have a proper cryonics transport box, because there was no time to get it there). > Finally, i think http://www.cryonics.org/research.html should also be > cited to vis-a-vis the ramp-up question. (I haven't reviewed this > yet, but i think it's clearly relevant in the decision making process > at CI that is referred to in the second quoted paragraph.) I have read the CI web page but have refrained from discussing it because I've been through this all before on CryoNet. I entered the vitrification debate only because I saw posts from Robert Ettinger that seemed, to me, to be misleading and incorrect. I have replied to those posts, and I have replied to his replies to my replies. The last thing I want right now is to start a new thread. My personal opinion (which has been stated many times before) is that any patient who is cryopreserved using standard CI procedures is unlikely to emerge from storage, under any circumstances, ever. I think maybe half of the patients at Alcor have some chance, depending on the circumstances of their deaths, and depending on future development of nanotechnology, which remains a totally speculative topic. Currently, I believe that if a patient is treated promptly after death, is transferred rapidly to the perfusion lab, and receives the most recent 21CM vitrification treatment, this person may have a relatively good chance of future resuscitation. However, there is NO instance in which ALL THREE of these requirements (prompt treatment after death, speedy transfer, and vitrification protocol) have been satisfied, even in the two most recent cases. The delivery of service remains a significant challenge, regardless of advances in vitrification capabilities. This is the main reason why I have not joined any of the currently active cryonics organizations myself. --CP Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=15259