X-Message-Number: 15278
Date: Sun, 7 Jan 2001 10:04:58 -0600 (CST)
From: "S.J. Van Sickle" <>
Subject: Fixation

On 7 Jan 2001, CryoNet wrote:

> From: Doug Skrecky <>
> Subject: chemical fixation

>  Frozen fixed tissue may exhibit increased intracellular ice crystal
> disruption, due to cell membranes being made too permeable, to
> allow freeze concentration mediated dehydration of cells.

If this is indeed the mechanism of increased damage to fixed, frozen
tissue, do you think that fixation and vitrification might be
complimentary techniques?

steve

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