X-Message-Number: 16251
Date: Thu, 10 May 2001 10:45:05 -0700 (PDT)
From: Doug Skrecky <>
Subject: HSCP -- Experiments and Accomplishments

>    In addition to the ice crystal damage of freezing and the toxicity of
cryoprotectant, attempts to cryopreserve organs & tissues are also hampered
by a mysterious phenomenon of unknown mechanism known as "chilling 
(1)   Chilling injury can be minimized by minimizing time spent at 0C.
      Chilling injury is confined to a window between +10C and -10C,
      being absent at both of these temperatures. 
I've read that chilling injury has been attributed to a temperature
dependant cell membrane phase transition. During this transition cell
contents can leak. This is why when dry yeast is hydrated, it has to be in
warm water.

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