X-Message-Number: 17210 Date: Mon, 6 Aug 2001 10:31:56 -0700 (PDT) From: Doug Skrecky <> Subject: CO2 versus LN2 > Message #17208 From: > "So how is Trygve's grandpa doing in the > shed with the dry ice?"..... > Dry ice may be an underrated short-term solution but is probably > insufficient for the multi-decade-long periods anticipated. > Here's a quote from "Long-term storage of Bone Marrow Cells at liquid Nitrogen and Dry Ice Temperatures" (Cryobiology 1: 65-69 1970): Summary: Bone marrow frozen with glycerol and stored at -69 to -79 C for 3 to 9 years was compared with bone marrow frozen with DMSO and stored for 3 years in liquid nitrogen (-120 to 196 C). It was found that bone marrow cells stored in Dry Ice were almost entirely destroyed after 3 years of storage while bone marrow cells in liquid nitrogen were largely intact after 3-year storage." Previous results of Dry ice storage were also mentioned. "It has been previously shown that bone marrow cells stored under identical conditions for periods of up to 1 year maintain their normal morphology. However chanmges become apparent by 2 years and were severe by 3." Reference for this was " Cytology of human bone marrow subjected to prolonged storage at -79 C" (J. Appl. Physiol. 19: 301-309 1964). I will let the readers decide for themselves about the probable condition of Trygve's grandpa. I will just mention that for less that the cost of setting up a dry ice storage containment facility independantly, Trygve could have stored his father's corpse in liquid nitrogen at CI. Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=17210