X-Message-Number: 17466 Date: Wed, 5 Sep 2001 20:43:56 -0700 (PDT) From: Doug Skrecky <> Subject: 45'th update on fly longevity experiments This is the 45'th update of my fly longevity experiments. All bottles contained 1/4 tsp citric acid as standard. Here I test a variety of vinegars at a lower 1 teaspoon level this time. Best result was with balsalmic vinegar, which offered superior survival for the first month. This vinegar had the darkest colour, and so I presume the highest phytochemical content. I note that this vinegar is manufactured from grape juice, and that grape juice has offered survival advantages on several occasions in the past when I have tested it. Since in humans potassium depletion is a side effect of excessive vinegar use, I decided to test the effect of a small nutritional supplement of potassium. This did appear to restore maximum survival of white vinegar treated flies to close to control levels. It is interesting that in a published study, which did not use an added acid, potassium also improved survival in flies. The next run will test the effect of various potassium salts. Run #45 Percent Survival on Day supplement 4 11 15 20 25 30 36 41 45 50 55 60 67 ___________________________________________________________________ control 100 88 76 64 60 56 56 56 48 36 24 8 0 vinegar, apple cider 1 tsp 96 78 74 65 52 35 22 4 4 0 - - - vinegar, balsalmic 1 tsp 100 100 94 94 89 72 50 44 17 0 - - - vinegar, malt 1 tsp 100 89 83 72 78 56 39 33 6 0 - - - vinegar, red wine 1 tsp 100 94 78 72 72 56 44 39 33 6 0 - - vinegar, rice 1 tsp 100 94 61 56 56 50 22 6 6 0 - - - vinegar, white 1 tsp 100 75 67 58 42 42 33 17 17 0 - - - + KHCO3 1/64 tsp 100 91 82 64 64 45 27 27 27 18 18 0 - Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=17466