X-Message-Number: 17713 Date: Sat, 6 Oct 2001 19:50:08 -0700 (PDT) From: Doug Skrecky <> Subject: 48'th update on fly longevity experiments This is the 48'th update of my fly longevity experiments. All bottles contain 1/4 tsp citric acid as a standard additive. Here I examine the effect of removing the corpses of dead flies at each census. Since I do not have access to CO2 gas, I placed the "no corpse" bottles in my fridge to temporarily reduce fly movement, so that corpses can be removed without live flies also escaping. One fly did escape during the day 9 census from the "no corpse 3" bottle, but the survival statistics were adjusted accordingly. Control bottles were also placed in the fridge as well at the same time so as to act as true controls. The population density in breeding bottles is much higher than in the bottles I use for longevity experiments. The risk of viral transmission would correspondingly be much higher in the breeding bottles. It makes sense that the presence of rotting fly corpses may aid in viral transmission. However it is not clear to me whether corpses are a risk to the few flies in each bottle of a longevity run. Here I decide the matter with an experiment. As can be seen in these results, removal of corpses has no effect on fly longevity. The variations in survival I attribute both to random variations in fly ages at the start of the experiment, and random variations in degree of viral infestation. In the next run I will examine the effect of various dietary fibers on longevity. Run #48 Percent Survival on Day supplement 5 9 14 19 24 31 36 42 47 52 57 63 _________________________________________________ control 1 94 82 82 82 ? 58 29 6 6 6 0 - control 2 95 84 68 58 ? 16 5 5 5 5 5 0 control 3 100 100 80 70 ? 55 30 25 10 0 - - no corpse 1 100 75 70 65 50 30 30 5 0 - - - no corpse 2 100 78 67 67 50 28 11 11 11 6 0 - no corpse 3 100 84 56 33 28 11 6 6 0 - - - Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=17713