X-Message-Number: 18111 Date: Fri, 7 Dec 2001 09:02:34 -0800 (PST) From: Doug Skrecky <> Subject: glycerol/borate combination for vitrification? Perfuse first with glycerol, then with borate. Like with trehalose/borate, I would expect the Tg' to be increased. Perhaps vitrification might occur with a lower concentration of glycerol? ---------- Forwarded message ---------- Title Glycerol-enhanced separation of DNA fragments in entangled solution capillary electrophoresis. Source Analytical Chemistry. 66(23):4210-4, 1994 Dec 1. Abstract (Hydroxyalkyl)methylcellulose additives to borate buffers have been used to create physically entangled solutions for fractionation of biological molecules by high-performance capillary electrophoresis, i.e., ESCE. We have observed that addition of glycerol to an entangled solution containing (hydroxypropyl)methylcellulose (HPMC) in Tris-borate buffer markedly improved the separation of double-strand DNA molecules ranging from less than 100 bp to about 1 kb by ESCE. This range of DNA fragment sizes is well suited to many PCR-based studies. We attribute the improved ESCE performance to the borate present in the buffer system. Borate can form dimeric 1:2 borate:didiol complexes with both glycerol and HPMC, acting as a central linkage to allow an entangled solution with different pore size to form chemically. Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=18111