X-Message-Number: 18321
Date: Wed, 9 Jan 2002 05:13:22 -0800 (PST)
From: Doug Skrecky <>
Subject: glycerol and dmso solution for lung cryopreservation

Title
  Ultrastructural and functional studies of cryopreserved rat lungs for
  transplantation using a new hyperosmolal solution.
Source
  Kaibogaku Zasshi - Journal of Anatomy.  75(3):275-83, 2000 Jun.
Abstract
  One of the most promising approaches for extending the period of tissue or
  organ preservation is to induce a state of cryopreservation. In this report,
  to achieve successful lung cryopreservation, we created a new hyperosmolal
  solution (HOS) containing 10% glycerol and 5%
  dimethyl sulfoxide (Me2SO). We compared the
  efficacy of HOS with that of Euro-Collins solution (ECS) in rat lung
  cryopreservation from an ultrastructural and functional point of view. Using
  ECS, widespread damage to the air-blood barrier was observed even in the
  lungs cryopreserved at -10 degrees C. At -196 degrees C, the capillary lumen
  was obstructed with a mesh-like formation derived from deteriorated
  endothelial cells. In contrast, using HOS, the cryopreserved lungs at -10
  degrees C were characterized by well preserved endothelial cells and basal
  laminae, despite the existence of focal cytoplasmic swelling of epithelial
  cells. The endothelial cells and basal laminae were successfully preserved up
  to -196 degrees C. Considering the survival rates at day 7 after
  cryopreserved lung transplantation, the grafted left lungs functioned
  normally only when perfused by and preserved in HOS at -10 degrees C. These
  findings demonstrate that HOS was effective not only for endothelial cells
  and basal laminae up to -196 degrees C, but also lung cryopreservation at
  least up to -10 degrees C.

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