X-Message-Number: 18421 From: Date: Sun, 27 Jan 2002 01:38:40 EST Subject: Cold Enough to Freeze the Ovaries Back On a Brass Rat? CryoNet: My thanks to Igor Artyuhov for pointing-out the PDF file pertaining to high pressure measures combined with temperature modifications for biological research endeavors as found in his last Tuesday's Post (Message #18392). I have an ongoing interest in this area for possible future work towards potentially reversible cryogenic suspended animation of small and/or simple life forms. I may eventually track down some or all of the documents referenced in that bibliography. For a repeat of the cited URL: http://www.hindawi.dk/journals/jbb/volume-1/S1110724301000158.pdf Also thanks to Jan Coetzee and Mike Perry for bringing attention to the article on functioning rat ovaries from liquid nitrogen. I believe that may be a particularly significant achievement. I hope the researchers do try other more sensitive organs from rats that may likewise be resuscitated. When we start hearing of kidneys, lungs, livers, etc., it might be time to make a few more Canadian researcher friends! I hope they consider pressure techniques in twinking protocol scenarios for the more challenging organs. If I am not mistaken, there appears to be at least one instance of mammalian organs' modulation of both pressure and temperature in the manufacturing and/or storage of proteins -- the testes for spermatozoa. I may attempt to figure out how to send these researchers the hyperlink on the pressure cooling/thawing just on the outside chance they are not aware of it or have not considered it just yet. A repeat of that URL is: http://www.nature.com/nsu/020121/020121-8.html A piece of interesting trivia for some: The rubbery whites of hard boiled eggs have semi-solidified from their former viscous liquid state due to the denaturing of their many tightly folded (i.e., balled-up) proteins. These huge molecules have unfolded under the heat and are now very long straight polypeptide "linear strings" that have randomly intertwine in all directions to form the resilient, tough gel-like structure of the cooked egg white. _____________________ On a total unrealted topic: I am certainly all in favor of as many individuals as are willing, to become involved in cryonics. However, while I may be missing something, it is not 100% obvious to me that the likely outcomes for current cryonicists are really heavily dependent on the increase in numbers to the movement. Just a thought. Regards, D.C. Johnson, Raleigh, NC Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=18421