X-Message-Number: 18915
From: "Smigrodzki, Rafal" <>
Subject: strain gauging in vitrification
Date: Thu, 11 Apr 2002 16:53:23 -0400

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I wonder if it might be possible to avoid brain fracturing during
vitrification by measuring the mechanical strain during cooling and
adjusting the rate to allow annealing. Since the fracturing occurs at a
rather low temperature, even extended annealing times might be feasible.
Inserting strain gauges before freezing would allow better monitoring of the
process.

Also, I wonder if it is possible to use perfusion solutions with
progressively lower freezing temps, up to the point where the tissue
vitrifies, while the vessels are still filled with fluid. If that is
possible (e.g. by using freons), then it might be realistic to accelerate
the rate of freezing without inducing high mechanical strain (by cooling
throughout the volume, in high pressure conditions to reduce the volume
expansion of the ice/vitrified water), and to allow thawing by perfusion
with a gradient of warming solutions.

Rafal

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