X-Message-Number: 18915 From: "Smigrodzki, Rafal" <> Subject: strain gauging in vitrification Date: Thu, 11 Apr 2002 16:53:23 -0400 This message is in MIME format. Since your mail reader does not understand this format, some or all of this message may not be legible. ------_=_NextPart_001_01C1E19A.EBB2A090 Content-Type: text/plain; charset="iso-8859-1" I wonder if it might be possible to avoid brain fracturing during vitrification by measuring the mechanical strain during cooling and adjusting the rate to allow annealing. Since the fracturing occurs at a rather low temperature, even extended annealing times might be feasible. Inserting strain gauges before freezing would allow better monitoring of the process. Also, I wonder if it is possible to use perfusion solutions with progressively lower freezing temps, up to the point where the tissue vitrifies, while the vessels are still filled with fluid. If that is possible (e.g. by using freons), then it might be realistic to accelerate the rate of freezing without inducing high mechanical strain (by cooling throughout the volume, in high pressure conditions to reduce the volume expansion of the ice/vitrified water), and to allow thawing by perfusion with a gradient of warming solutions. Rafal ------_=_NextPart_001_01C1E19A.EBB2A090 Content-Type: text/html; [ AUTOMATICALLY SKIPPING HTML ENCODING! ] Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=18915