X-Message-Number: 18963
From: "brent thomas" <>
Subject: experimental results: green tea extract increases graft survival rates
Date: Wed, 24 Apr 2002 17:47:34 -0400

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theres an interesting article at 
http://www.sciencedaily.com/releases/2002/04/020423080518.htm detailing some 
liver transplantation experiements in rats. Its a given that rat based 
experiments are different than human ones in results and magnitudes but still 
this has some interesting facets.


basically the interesting part of it is in transplanted livers damaged by 
alcohol (and isnt alcohol in many ways similar to the preserving components of 
perfusates?) the use of green tea extracts (epigallocatechin gallate) blunted 
graft trauma. They suspect this was due largely to its scavenging ability 
against free radicals caused by the trauma.


This got me thinking that in a cryonics attempt (perfuse, freeze, rewarm) there 
is probably a lot of cellular level trauma and I wondered what if any attempts 
had been made to measure the effects of free radical scavengers added to a 
perfusate in terms

of the viablity of the sample after rewarming? it doesnt seem that it could hurt
the process (unless the scavenger percentage in the solution caused the 
preserving ability of the solution to be decreased)...


anyone know if any attempts have been made to 'load' perfusate with free radical
scavenging materials and if this had any effect on the survival or viability 
percentages?

some interesting extracts from the article
>>>>>>>>>>>>>>>>>>>>.

 Green tea extract largely blunted graft injury and increased survival of fatty 
 livers to 75 percent. 


Ethanol administered to donor rats increased POBN/radical adducts 2.5-fold and 
caused accumulation of 4-hydroxynenonal, a product of lipid peroxidation, after 
transplantation. These effects were largely blocked by green tea extract or 
epigallocatechin gallate, a major polyphenolic component of green tea extract. 
>>>>>>>>>>>>>>>>>>>


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