X-Message-Number: 18963 From: "brent thomas" <> Subject: experimental results: green tea extract increases graft survival rates Date: Wed, 24 Apr 2002 17:47:34 -0400 This is a multi-part message in MIME format. ------=_NextPart_000_0005_01C1EBB8.1D4DEAC0 Content-Type: text/plain; charset="iso-8859-1" theres an interesting article at http://www.sciencedaily.com/releases/2002/04/020423080518.htm detailing some liver transplantation experiements in rats. Its a given that rat based experiments are different than human ones in results and magnitudes but still this has some interesting facets. basically the interesting part of it is in transplanted livers damaged by alcohol (and isnt alcohol in many ways similar to the preserving components of perfusates?) the use of green tea extracts (epigallocatechin gallate) blunted graft trauma. They suspect this was due largely to its scavenging ability against free radicals caused by the trauma. This got me thinking that in a cryonics attempt (perfuse, freeze, rewarm) there is probably a lot of cellular level trauma and I wondered what if any attempts had been made to measure the effects of free radical scavengers added to a perfusate in terms of the viablity of the sample after rewarming? it doesnt seem that it could hurt the process (unless the scavenger percentage in the solution caused the preserving ability of the solution to be decreased)... anyone know if any attempts have been made to 'load' perfusate with free radical scavenging materials and if this had any effect on the survival or viability percentages? some interesting extracts from the article >>>>>>>>>>>>>>>>>>>>. Green tea extract largely blunted graft injury and increased survival of fatty livers to 75 percent. Ethanol administered to donor rats increased POBN/radical adducts 2.5-fold and caused accumulation of 4-hydroxynenonal, a product of lipid peroxidation, after transplantation. These effects were largely blocked by green tea extract or epigallocatechin gallate, a major polyphenolic component of green tea extract. >>>>>>>>>>>>>>>>>>> ------=_NextPart_000_0005_01C1EBB8.1D4DEAC0 Content-Type: text/html; [ AUTOMATICALLY SKIPPING HTML ENCODING! ] Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=18963