X-Message-Number: 19489 Date: Fri, 12 Jul 2002 08:14:06 -0700 (PDT) From: Doug Skrecky <> Subject: 62'nd update on fly longevity experiments This is the 62'nd update of my fly longevity experiments. Average temperature was 22.2 C during this run. Citric acid is a standard additive to all runs, since it helps to inhibit mold, prevents color changes in the fly food (keeping it 'fresh'), and increases fly longevity. In this run I look at the interaction between citric acid and supplements with antiviral activity. The mold inhibiting effect of citric acid proved its worth again. Although standard 4-24 fly food contains a mold inhibitor, I have found this not to be fully effective. Mold appeared in the citric-acid-free soy/rice protein bottle, and increased mortality possibly by "drowning" some of the flies. Freeze concentrated Knudsen Elderberry juice increased survival compared to the acid free control bottle, but not significantly in comparison to citric acid by itself. I suspect, possibly due to the relatively low ambient temperature, that virus infections might not have increased mortality very much in this run. Once again adding 2 teaspoons of dry protein without additional water decreased maximum longevity, probably by dehydration stress. Run #62 Percent Survival on Day supplement 15 20 29 36 41 46 51 61 67 72 80 87 93 ________________________________________________________________ control 93 93 86 79 79 64 50 21 14 7 0 - - elderberry FC 92 92 92 92 92 85 77 62 38 31 31 15 0 soy/rice protein 2 tsp 100* 89 50 33 22 22 11 6 6 6 0 - - elderberry FC & soy/rice 93 93 93 93 93 80 80 67 40 13 13 0 - citric acid 1/4 tsp 100 100 86 86 86 86 71 71 50 14 7 0 - + elderberry FC 93 93 93 87 87 87 87 67 47 27 13 0 - + soy/rice 2 tsp 93 93 93 93 93 87 67 27 7 0 - - - + elderberry & soy/rice 91 91 82 82 82 82 64 55 36 0 - - - *Mold appeared in the citric acid free soy/rice bottle. Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=19489