X-Message-Number: 19582 Date: Sun, 21 Jul 2002 11:15:35 -0700 (PDT) From: Doug Skrecky <> Subject: the early history of vitrification Looks like Elford & Walter had vitrification pretty much figured out 30 years ago. All muscles were incubated for 60 min at +37 C in solutions containing 20% (w/w) DMSO. Interesting that all ability to contract is suppressed during contact with much lower concentrations of DMSO. Subsequent incubation was in various different solutions containing 30, 40, 50, and 60% (w/v) DMSO at temperatures of -7, -14, -22, and -39 C respectively [for 1 hr at each temperature], before they were finally cooled to -79 C and left overnight. Some very good results were obtained with low pH PIPES. The 1978 paper further builds on these interesting results. ---------- Forwarded message ---------- Citations: 1-2 <1> Authors Taylor MJ. Walter CA. Elford BC. Title The pH-dependent recovery of smooth muscle from storage at -13 degrees C in unfrozen media. Source Cryobiology. 15(4):452-60, 1978 Aug. <2> Authors Elford BC. Walter CA. Title Effects of electrolyte composition and pH on the structure and function of smooth muscle cooled to -79 degrees C in unfrozen media. Source Cryobiology. 9(2):82-100, 1972 Apr. Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=19582