X-Message-Number: 20137 Date: Sun, 22 Sep 2002 11:24:25 -0700 (PDT) From: Doug Skrecky <> Subject: Lemon oil as a possible additive to cryoprotectant solutions? From: mike Newsgroups: alt.baldspot : Pharmacol Res 2002 Jun;45(6):469 Inhibitory effect of apoptosis in human astrocytes CCF-STTG1 cells by lemon oil. Koo H, Hong S, Kim C, Ahn J, Lee Y, Kim J, Lyu Y, Kim H. Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University, KI Co. Ltd. Iksan, Chonbuk, 570-749, Republic of Korea The effects of lemon pure essential oils on the heat shock-induced apoptosis in human astrocytes cell line CCF-STTG1 were examined. In previous studies, heat shock has been reported to induce the apoptosis or programmed cell death through the activation of caspase-3. Treatment of heat shock on CCF-STTG1 cells markedly induced apoptotic cell death as determined by flow cytometry. Interestingly, pre-treatment with lemon pure essential oils on CCF-STTG1 cells inhibited the heat shock-induced apoptosis. Lemon oil also inhibited the heat shock-induced apoptosis in primary cultured rat astrocytes. To determine whether lemon oil inhibits the heat shock-induced activation of the apoptotic proteases, activation of caspase-3 was assessed by Western blotting. DNA fragmentation, giemsa staining, and heat shock-induced activation of caspase-3 were blocked by lemon pure essential oil, which is consistent with flow cytometry. Poly-ADP-ribose polymerase (PARP), the cysteine protease substrate, was fragmented as a consequence of apoptosis by heat shock. Lemon oil inhibited the PARP fragmentation. These results suggest that lemon pure essential oils may modulate the apoptosis through the activation of the interleukin-1 beta -converting enzyme-like caspases. """""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""" : Drugs Exp Clin Res 1999;25(6):281-7 Oxidative stress and antioxidants at skin biosurface: a novel antioxidant from lemon oil capable of inhibiting oxidative damage to the skin. Calabrese V, Scapagnini G, Randazzo SD, Randazzo G, Catalano C, Geraci G, Morganti P. Faculty of Medicine, Department of Chemistry, University of Catania, Italy. Atmospheric pollutants are an important source of oxidative and nitrosative stress both to terrestrial plants and to animals. Skin, which has a highly differentiated and certainly complex organizational structure, is particularly vulnerable to free radical damage because of its contact with oxygen and with other environmental stimuli. Fruit and vegetables contain several classes of compounds that when ingested can potentially contribute to antioxidant defenses. In the present study we employed a novel gas chromatographic method to assess the antioxidant properties of a natural compound isolated from lemon oil, which we have called Lem1. We provide experimental evidence that Lem1 is endowed with a strong antioxidant activity and that it is capable of inhibiting free radical-mediated reactions, as evaluated in vitro and in vivo. The present study extends our previous findings and demonstrates that topical application of Lem1 in healthy volunteers significantly increases the antioxidative potential of skin biosurface, thus highlighting the effectiveness of a natural antioxidant biotechnology in the antiaging management of skin. Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=20137