X-Message-Number: 21745
Date: Sun, 11 May 2003 11:26:08 -0700 (PDT)
From: Doug Skrecky <>
Subject: ideas

I know ideas are a dime a dozen, but here's a couple more anyhow.

#1. Preperfusing with a cocktail of chemicals to protect against
cryoprotectant toxicity. (perhaps easier said than done)
[Examples:
Isoflurane protects against glycerol induced renal failure -
J Am Soc Nephrol 9: 305-309, 1998,
or use Taxol, a cytoskeleton stabilizer -
Fertility and Sterility June 2001 Vol.75 No.6 1177-1184
or even natural honey protects against ethanol damage -
Journal of Ethnopharmacology 1997 55: 231-238
or ursodeoxycholate - Life Sciences 1998 Vol.63 No.25 2259-2270 1998
or glucosamine - Animal Reproduction 2002 Voo.69: 91-97
or trehalose versus DMSO - Diabetes 1997 46: 519-523
etc, etc]

#2. Preperfusing with obscure agents to protect against anoxia damage.
(example: DMSO and fructose 1,6 phosphate)

#3. Try less damaging cryoprotectants.
[Example:
Triethylene glycol is much less toxic than either diethylene glycol or
ethylene glycol -
Vet  Hum Toxicol 2001 Oct; 43(5):269-76
or Urea less toxic to brain than glycerol
Stroke 1983 Vol.14 No.4 597-604]

#4. Rely more on dehydration, and less on infusion of toxic
cryoprotectants.

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