X-Message-Number: 21745 Date: Sun, 11 May 2003 11:26:08 -0700 (PDT) From: Doug Skrecky <> Subject: ideas I know ideas are a dime a dozen, but here's a couple more anyhow. #1. Preperfusing with a cocktail of chemicals to protect against cryoprotectant toxicity. (perhaps easier said than done) [Examples: Isoflurane protects against glycerol induced renal failure - J Am Soc Nephrol 9: 305-309, 1998, or use Taxol, a cytoskeleton stabilizer - Fertility and Sterility June 2001 Vol.75 No.6 1177-1184 or even natural honey protects against ethanol damage - Journal of Ethnopharmacology 1997 55: 231-238 or ursodeoxycholate - Life Sciences 1998 Vol.63 No.25 2259-2270 1998 or glucosamine - Animal Reproduction 2002 Voo.69: 91-97 or trehalose versus DMSO - Diabetes 1997 46: 519-523 etc, etc] #2. Preperfusing with obscure agents to protect against anoxia damage. (example: DMSO and fructose 1,6 phosphate) #3. Try less damaging cryoprotectants. [Example: Triethylene glycol is much less toxic than either diethylene glycol or ethylene glycol - Vet Hum Toxicol 2001 Oct; 43(5):269-76 or Urea less toxic to brain than glycerol Stroke 1983 Vol.14 No.4 597-604] #4. Rely more on dehydration, and less on infusion of toxic cryoprotectants. Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=21745