X-Message-Number: 2191
From:  (Will Dye)
Newsgroups: sci.cryonics
Subject: Re: C. elegans freezing protocol. Please comment
Date: 1 May 1993 19:42:10 GMT
Message-ID: <>
References: <>

Quick suggestions:

1.  More control groups.  Nematodes that weren't frozen at
all (to prove the group was normal), some that were 
frozen in a manner more like the onset of winter (to 
compare with animals that routinely freeze), etc.
Nemo's are cheap, and even when control groups aren't 
really necessary, they look good when it comes time 
to defend your conclusions.  

2.  What's your methodology for measuring neural damage?  
I guess you mean you'll try the dish-tapping routine, but 
I don't recall seeing that in your post.  See if you can 
finangle several types of tests, instead of just one.  

3. If it's not too hard, try proving that a damaged nemo 
neural net wouldn't function by referring to the 
neural maps that are already available (i.e. simulate 
an uploaded nemo on the computer, assessing how much 
damage the net can take and still perform as observed).
I realize this is too much to do by yourself, but check 
to see if somebody has already set up the program for 
you.

--Will

P.S.  BTW, how well do E. Coli freeze? 

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