X-Message-Number: 2191 From: (Will Dye) Newsgroups: sci.cryonics Subject: Re: C. elegans freezing protocol. Please comment Date: 1 May 1993 19:42:10 GMT Message-ID: <> References: <> Quick suggestions: 1. More control groups. Nematodes that weren't frozen at all (to prove the group was normal), some that were frozen in a manner more like the onset of winter (to compare with animals that routinely freeze), etc. Nemo's are cheap, and even when control groups aren't really necessary, they look good when it comes time to defend your conclusions. 2. What's your methodology for measuring neural damage? I guess you mean you'll try the dish-tapping routine, but I don't recall seeing that in your post. See if you can finangle several types of tests, instead of just one. 3. If it's not too hard, try proving that a damaged nemo neural net wouldn't function by referring to the neural maps that are already available (i.e. simulate an uploaded nemo on the computer, assessing how much damage the net can take and still perform as observed). I realize this is too much to do by yourself, but check to see if somebody has already set up the program for you. --Will P.S. BTW, how well do E. Coli freeze? Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=2191