X-Message-Number: 23018
From: "Basie" <>
Subject: post-mortem brain.
Date: Thu, 4 Dec 2003 21:20:51 -0500

      1: Brain Res Mol Brain Res. 1995 Feb;28(2):311-8.  Related Articles,

Tissue pH as an indicator of mRNA preservation in human post-mortem brain.

Kingsbury AE, Foster OJ, Nisbet AP, Cairns N, Bray L, Eve DJ, Lees AJ,
Marsden CD.

MRC Human Movement and Balance Unit, Institute of Neurology, London, UK.

The relationship between pH and mRNA preservation in post-mortem human brain
was examined using in situ hybridization histochemistry and Northern
hybridization with oligonucleotide probes in a large group of human
subjects, including control and neuropathological cases. Tissue pH was found
to correlate strongly with preservation of four mRNA species in three brain
areas. Tissue with low pH, assumed to result from prolonged terminal
hypoxia, contained reduced or absent mRNA, while tissue with higher pH was
found to contain quantifiable amounts, the values for pathological brain
samples being comparable to those for control material of similar pH.
Measurement of tissue pH provides a simple means to screen post-mortem brain
for mRNA preservation and is suggested as a means to match material in
case-control studies of human neurodegenerative disease.

PMID: 7723629 [PubMed - indexed for MEDLINE]



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