X-Message-Number: 24466
Date: Wed, 4 Aug 2004 09:49:58 -0700 (PDT)
From: Doug Skrecky <>
Subject: substituting ETSO for DMSO in vitrification solutions?

Message #24460 From: Thomas Donaldson <> wrote:
> Unfortunately DMSO has been thoroughly investigated for a long time
> as a cryoprotectant and doesn't work as well as some others. I am
> not engaged in studying cryoprotectants, I'm just reporting what
> I've read. Greg Fahy, who counts as an authority on this subject,
> is probably much too busy with vitrification to reply to your
> message.
?? The article I posted was about ETSO, not DMSO.
Greg has recently found that vitrification solution toxicity was reduced
when the propylene glycol component was substituted by ethylene glycol.
This new article suggests that a further substitution of the
dimethylsulfoxide (DMSO) component by diethylsulfoxide (ETSO) would be
worth testing.

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