X-Message-Number: 24490 Date: Mon, 9 Aug 2004 20:00:02 -0700 (PDT) From: Doug Skrecky <> Subject: 146'th update on fly longevity experiment This is the 146'th update of my fly longevity experiments. Average temperature was 25.3 C during this run. Estimated maximal longevity using the formula (363 - T*11.2) is 80 days. In this run I take a look again at doses of citric acid higher than the control 1/2 tsp, and also check out lactobacillus "probiotic" bacteria (75% acidophilus & 25% bifidus). Citric acid at 1 & 2 tsp doses appeared to help. Lactobacillus was also beneficial, but only at doses much larger than those that were immunostimulatory in mammalian species. Run #146 Percent Survival on Day supplement 25 30 35 40 52 61 67 73 78 83 88 93 ________________________________________________________________ control one 100 100 94 91 71 35 35 12 6 0 - - control two 100 100 100 98 70 28 18 0 - - - - alum 1/8 tsp 91 82 71 61 36 11 2 0 - - - - alum 1/2 tsp 0 - - - - - - - - - - - blackberry syrup 1/2 tsp 100 100 97 97 71 40 32 5 0 - - - blackberry 2 tsp 95 95 95 92 78 41 24 11 8 0 - - citric acid 1 tsp 100 100 100 95 81 61 51 12 0 - - - citric acid 2 tsp 100 95 95 95 85 50 33 13 5 3 0 - citric acid 3 tsp 100 94 94 88 65 29 21 6 0 - - - lactobacillus 4 bil cells 100 95 88 81 62 33 26 10 3 0 - - lactobacillus 8 bil cells 90 90 90 90 77 43 33 17 3 0 - - lactobacillus 16 bil cells 96 96 89 75 64 39 36 7 7 4 0 - lactobacillus 32 bil cells 92 92 84 84 60 48 36 12 8 8 4 0 Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=24490