X-Message-Number: 24547 Date: Mon, 23 Aug 2004 05:16:13 -0700 (PDT) From: Doug Skrecky <> Subject: 145'th update onfly longevity experiments (I was told the 145'th update never reached this list.) ---------- Forwarded message ---------- This is the 145'th update of my fly longevity experiments. Average temperature was 25.2 C during this run. Estimated maximal longevity using the formula (363 - T*11.2) is 81 days. This run looks at some doses of amino acids that are higher than those tested before. Control longevity was excellent in this run. This might be due to the increase in citric acid from 1/8 tsp to 1/2 tsp in all bottles. Sucrose 1 tsp was added as well. Although some amino acids were toxic when added in excess, at least leucine, and isoleucine appeared to be safe. Arginine looked good during the first month, though survival subsequently tailed off. This might be due to the surface wetness induced in the fly food by the added arginine. At young ages, flies walk mostly on the sides of the milk bottle. As they age they spend much more time walking on the fly food at the bottom of the bottles. The wetness of arginine laced food, may have made the aged flies dirty, and encouraged bacterial infections. Run #145 Percent Survival on Day supplement 7 12 21 26 31 36 41 53 62 68 74 79 84 ____________________________________________________________ control one 100 100 100 100 100 97 85 55 15 6 3 0 - control two 100 100 100 98 98 96 93 64 24 22 20 4 0 alanine 2 tsp 100 85 6 0 - - - - - - - - - arginine 2 tsp 100 100 100 100 100 84 69 19 3 0 - - - cysteine 2 tsp 0 - - - - - - - - - - - - glycine 2 tsp 59 32 0 - - - - - - - - - - isoleucine 2 tsp 100 100 96 96 96 93 89 57 43 18 4 0 - leucine 2 tsp 100 100 96 96 96 96 92 52 32 20 4 0 - taurine 2 tsp 97 90 71 55 55 53 42 8 0 - - - - threonine 2 tsp 57 0 - - - - - - - - - - - valine 2 tsp 100 100 100 93 83 43 3 0 - - - - - Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=24547