X-Message-Number: 24557
Date: Thu, 26 Aug 2004 00:19:15 -0700 (PDT)
From: Doug Skrecky <>
Subject: ethylene glycol & brain tissue

Peptides. 1986 Jan-Feb;7(1):155-9. Erratum in: Peptides 1986 May-Jun;7(3):545.
Use of cryoprotectant to maintain long-term peptide immunoreactivity and
tissue morphology.
  Use of an ethylene glycol based cryoprotectant solution has been found
to be effective for the long-term storage of brain tissue either in block
form or as freely floating sections prior to immunocytochemical
processing. Storage of tissue in the solution at -20 degrees C or 4
degrees C for up to 3 months produced no adverse effects upon tissue
morphology, nor was LHRH immunoreactivity diminished or accompanied by
elevated non-specific staining. Furthermore, ultrastructural analysis of
cryoprotected tissue revealed excellent preservation of cellular
morphology. It is anticipated that this method can find use when it is
necessary or desirable for the investigator to retain tissue for later
immunocytochemical or electron microscopic processing.

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