X-Message-Number: 24565
Date: Sat, 28 Aug 2004 17:28:53 -0700 (PDT)
From: Doug Skrecky <>
Subject: effect of different cryoprotectants

Theriogenology. 2004 Apr 15;61(6):1101-14.
Effect of different cryoprotectants on the structural preservation of
follicles in frozen zebu bovine (Bos indicus) ovarian tissue.
  Cryopreservation of ovarian tissue is a new and promising technique for
germ-line storage. The objective of this study was to evaluate the effect
of four cryoprotectants (at two concentrations each) on the preservation
of zebu bovine preantral follicles after ovarian cryostorage. Strips of
ovarian cortex were cryopreserved using glycerol (GLY; 10 or 20%),
ethylene glycol (EG), propanediol (PROH) or dimethylsulphoxide (DMSO; 1.5
or 3M). In addition, a toxicity test was performed for each
cryoprotectant by exposing the ovarian tissue to them without freezing.
Tissues were analyzed by histology and transmission electron microscopy.
Ovarian tissue frozen in either concentration of DMSO or PROH or in 10%
GLY retained a higher percentage of morphologically normal follicles
(73-88%) than tissue frozen in 20% GLY or in either concentration of EG
(16-52%). In the toxicity test, exposure of tissues to DMSO, PROH or GLY
resulted in higher percentages of normal follicles (80-97%) than exposure
to EG (49%). Electron microscopy revealed damage to the ultrastructure of
follicles frozen in 10% GLY, while follicles cryopreserved in DMSO and
PROH at either concentration exhibited normal ultrastructure. In
conclusion, DMSO and PROH were the most effective cryoprotectants for
zebu ovarian tissue, preserving the structural integrity of somatic and
reproductive cells within the ovary.

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