X-Message-Number: 24568
Date: Mon, 30 Aug 2004 17:54:18 -0700 (PDT)
From: Doug Skrecky <>
Subject: naringin versus glycerol toxicity

Toxicology. 2004 Sep 1;201(1-3):143-51
Protective effect of naringin, a bioflavonoid on glycerol-induced acute
renal failure in rat kidney.
  Rhabdomyolysis-induced myoglobinuric acute renal failure accounts for
about 10-40% of all cases of acute renal failure (ARF). Reactive oxygen
intermediates have been demonstrated to play an etiological role in
myoglobinuric renal failure. This study was designed to investigate the
effect of naringin, a bioflavonoid with antioxidant potential, in
glycerol-induced ARF in rats. Five groups of rats were employed in this
study, group I served as control, group II was given 50% glycerol
(8ml/kg, intramuscularly), group III, IV, and V were given glycerol plus
naringin 100, 200, and 400mg/kg p.o. route, respectively) 60min prior to
the glycerol injection. Renal injury was assessed by measuring plasma
creatinine, blood urea nitrogen, creatinine, and urea clearance. The
oxidative stress was measured by renal malondialdehyde levels, reduced
glutathione levels, and by enzymatic activity of catalase, glutathione
reductase, and superoxide dismutase. Glycerol treatment resulted in a
marked renal oxidative stress and significantly deranged the renal
functions. Pretreatment of animals with naringin 60min prior to glycerol
injection markedly attenuated renal dysfunction, morphological
alterations, reduced elevated thiobarbituric acid reacting substances
(TBARS), and restored the depleted renal antioxidant enzymes. These
results clearly demonstrate the role of oxidative stress and its relation
to renal dysfunction, and suggest a protective effect of naringin in
glycerol-induced renal failure in rats

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