X-Message-Number: 24664 Date: Sun, 19 Sep 2004 12:45:42 -0700 (PDT) From: Doug Skrecky <> Subject: 152'nd update on fly longevity experiments This is the 152'nd update of my fly longevity experiments. Average temperature was 26.8 C during this run. Estimated maximal longevity (EML) using the formula (363 - T*11.2) is 63 days. Here I have a ube showdown. Back in run #138, raw purple sweet potato (ube) appeared to be beneficial. However ube was a dud in run #148 in powder form, and in run #149 in frozen form. The processing of later two forms would have involved cooking, which might be the critical difference. Here I check ube that is raw, as well as boiled whole. I also test the effect of cutting the ube into small pieces, so that boiling can then leach much of the purple color. Finally a hot water extract of ube is also tested. By a fluke of luck the control one bottle's maximum survival exeeded EML by 17%. As expected, boiling the ube rendered it mildly toxic. Whatever was responsible for this toxicity, it was poorly water soluble, since most of the hot water extracts slightly increased survival at 52 days. In published experiments the heating of fats has increased their toxicity to flies. This might account for the current results. Raw ube proved to beneficial again. My primary suspects for ube's main prolongevity ingrediants are elements of the anthocyanidin-based purple coloring. Run #152 Percent Survival on Day supplement 11 20 26 32 37 42 47 52 58 63 68 74 79 ________________________________________________________ control one 93 90 90 79 68 57 39 18 14 11 7 4 0 control two 97 89 89 83 69 49 34 20 14 0 - - - ube, leached 20% 100 51 51 31 26 3 0 - - - - - - ube, leached 20% 97 79 73 52 36 15 3 0 - - - - - ube, boiled 20% 92 77 59 28 8 0 - - - - - - - ube, boiled 20% 100 93 89 63 44 33 7 4 0 - - - - ube, extract 20% 97 93 93 93 90 73 53 27 7 3 0 - - ube, extract 20% 92 89 89 85 81 62 42 15 0 - - - - ube, extract 50% 100 96 92 84 64 60 40 24 8 0 - - - ube, extract 50% 100 91 91 83 74 57 39 26 9 4 0 - - ube, raw 20% 100 81 81 81 69 63 44 38 13 0 - - - ube, raw 20% 97 93 93 93 79 61 54 50 18 4 0 - - Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=24664