X-Message-Number: 25787 Date: Sun, 13 Mar 2005 12:35:59 -0800 (PST) From: Doug Skrecky <> Subject: is policosanol's cholesterol lowering effect a hoax? [According to a Cuban group of researchers sugar cane derived policosanol is more effective than most statins for lowering cholesterol. Unfortunately Cuban policosanol is under embargo, so only policosanol from other sources can be independantly tested. The clinical results using these alternative sources such as rice, sunflower seed, wheat as well as sugar cane, all indicate that policosanol has no effect on cholesterol.] Metabolism. 2004 Oct;53(10):1309-14 Wheat germ policosanol failed to lower plasma cholesterol in subjects with normal to mildly elevated cholesterol concentrations. Sugar cane policosanol, a mixture of long-chain primary alcohols (approximately 67% as octacosanol), has been reported to lower plasma low-density lipoprotein (LDL)-cholesterol. We investigated the effect of wheat germ policosanol (WGP) on plasma lipid profiles in 58 adults (30 men and 28 women, aged 49 +/- 11 years) with normal to mildly elevated plasma cholesterol concentrations in a double-blind, randomized, parallel placebo-controlled study. Subjects consumed chocolate pellets with or without 20 mg/d WGP for 4 weeks. Plasma lipid concentrations, routine blood chemistry and hematology were determined at the start and the end of the study. The initial plasma total, LDL-cholesterol, high-density lipoprotein (HDL)-cholesterol,and triacylglycerol concentrations in the WGP and the control groups were identical. Over the 4 weeks, neither the WGP nor the control treatment significantly changed plasma total cholesterol, LDL- and HDL-cholesterol, or triacylglycerol concentrations when compared to baseline values. In addition, there was no significant difference in plasma lipid profiles between the WGP and the control groups at the end of the study. WGP did not result in any adverse effects as indicated by plasma activities of L-gamma-glutamyltransferase (gamma-GT), ALT, AST, bilirubin concentrations, and blood cell profiles. Chemical analysis showed that WGP consists of 8% hexacosanol, 67% octacosanol, 12% triacosanol, and 13% other long-chain alcohols, which is similar to the composition of sugar cane policosanol. In conclusion, WGP at 20 mg/d had no beneficial effects on blood lipid profiles. It therefore seems unlikely that the long chain (C24-34) alcohols have any cholesterol-lowering activity. Asia Pac J Clin Nutr. 2004 Aug;13(Suppl):S69. Lack of effect of sugar cane and sunflower seed policosanols on plasma cholesterol in rabbits. Background- Policosanol, a mixture of high molecular weight primary aliphatic alcohols from sugar cane (SCP), has been shown to be safe and effective at lowering blood cholesterol when administered in low (pharmacological) doses (5-20 mg/day) to experimental animals, healthy patients and patients with type II hypercholesterolemia. Objective- We attempted to see whether a similar product derived from winteriser cake containing policosanols during sunflower oil production (SFP) has cholesterol-lowering potential. Design- Normocholesterolemic rabbits were administered either a control oil (water/emulsifier/sunflower oil emulsion), 100 mg/kg SCP (Lesstanol(R), provided by Johnson & Barana) (in control) or 100 mg/kg SFP winteriser cake (provided by Goodman Fielder) (in control) by gavage at 48 hour intervals for 4 weeks. Fasting blood samples were taken for cholesterol (C) and triglyceride (TAG) analysis at weekly intervals from 1week beforehand. Outcomes- The table shows changes in plasma lipids between averaged pre- and post-treatment values. Food intake and body weight were unaffected by the treatments. Plasma low-density lipoprotein (LDL)-C increased and plasma TAG decreased in all groups following gavaging: hence there was no treatment effect of either policosanol. Conclusions- Our data do not confirm a hypocholesterolemic effect of policosanols extracted from either sunflower oil cake or sugar cane, even though a lower dose of SCP than used here (5 mg /day) has been reported to lower cholesterol in rabbits. Lipids. 2003 Feb;38(2):165-70. Effects of policosanols and phytosterols on lipid levels and cholesterol biosynthesis in hamsters. The current study was carried out to examine the effects of policosanols and phytosterols, alone and in combination, on lipid profiles, cholesterol biosynthesis, and tissue histopathological changes in hamsters. Fifty male Golden Syrian hamsters, weighing 100 to 120 g, were fed a regular rodent chow for 2 wk before being randomly assigned into 5 groups of 10 animals each fed semisynthetic diets for 4 wk. Group 1 was given a control diet that contained 0.25% cholesterol and 5% fat with a PUFA to saturated FA ratio of 0.4. Groups 2 to 5 were fed the control diet and given Octa-6 [a policosanol mixture from sugar cane wax, 25 mg/kg body weight (BW)], Ricewax (a policosanol mixture from rice wax with 50% being converted to the corresponding acids, 50 mg/kg BW), phytosterols (Cholestatin; 1,000 mg/kg BW), and Ricewax (50 mg/kg BW) plus phytosterols (1,000 mg/kg BW), respectively. The results showed that there was no difference between Octa-6 and Ricewax treatments in any of the lipid parameters measured, and both had similar levels of triglyceride (TG), total cholesterol (T-C), and HDL cholesterol (HDL-C) as the control. Octa-6 but not Ricewax increased (P = 0.03) non-HDL-C as compared with the control. Phytosterols reduced T-C (P < 0.0003) and HDL-C (P < 0.004) without a significant effect on TG and non-HDL-C as compared to the control. Ricewax plus phytosterols had effects similar to those with phytosterols alone. Free cholesterol synthetic rates were not different among the treatments. Policosanols or phytosterols did not show any toxic effects in liver, heart, brain, or kidney. Results suggest that, although phytosterols reduce T-C and HDL-C levels, policosanols have no significant favorable effect in changing lipid levels in hamsters. Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=25787